山东省HCV分离株C区及NS5区核苷酸序列分析及其基因分型  

Analysis of the nucleotide sequence for C and NS5 regions and the genotype of HCV isolate in Shandong Province

作　　者：张文卿[1] 于红[1] 王笑峰[1] 王芹[1] 吕锐[1] 王海青[1] 邵济钧[1] 

机构地区：[1]青岛大学医学院分子病毒学重点实验室,青岛266021

出　　处：《中华实验和临床病毒学杂志》2001年第3期219-221,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省科委资助基金 (96 12 2 6 72 2 )

摘　　要：目的　研究山东省丙型肝炎病毒 (HCV)流行株的基因型别。方法　用反转录套式聚合酶链反应 (PCR)方法分别扩增山东省HCV流行株C区 (4 32bp)NS5区 (319bp)的两个基因片段 ,将其克隆入T载体上并自动测序 ,进行同源性分析及基因型别鉴定。结果　 4株HCVC区基因片段有 3株为 1b基因亚型 ,1株为 2a基因亚型 ,10株NS5区的基因片段分析均为 1b基因亚型 ,并且与GenBank中多个 1b亚型代表株核苷酸序列同源性达 90 %以上。结论　山东省HCV流行株以 1b亚型为主 ,兼有 2a亚型。同一亚型中也有较大的变异 ,NS5区 1b亚型中基因离散率可达 5 %以上。Objective To study the genotype of HCV epidemic strains in Shandong Province.Methods HCV fragments of C(432 bp)and NS5(319 bp) regions were amplified by RT nest PCR.The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction.Results Three of all 4 strains in C region were 1b genotye,and 1 was 2a.All of 10 strains of NS5 region were 1b genotype.No other genotype was found in this region.The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in GenBank.Conclusion The major genotype of HCV epidemic strains in Shandong Province is 1b.The 2a genotype is also found in this area.

关 键 词：丙型肝炎病毒 碱基序列 基因型 山东 

分 类 号：R373[医药卫生—病原生物学]