HIV1膜蛋白PND编码基因自然变异株的发现  

Discovery of natural variant strain of HIV1 in env gene C2- V3 encoded PND of membrane protein

作　　者：王斌[1] 傅继华[2] 吴虹[1] 刘传新[2] 钱冬萌[1] 方荣[1] 

机构地区：[1]青岛大学医学院分子病毒学实验室,青岛266021 [2]山东省防疫站

出　　处：《中华实验和临床病毒学杂志》2001年第3期245-247,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：卫生部科研基金 (96 13 5 3 ) ;山东省优秀中青年科学家奖励基金 (973 14 4 3 2 )

摘　　要：目的　对 1例来自华东地区HIV1分离株 (WWBH7)的前病毒env基因C2 V3区进行序列分析。方法　以HIV1感染者外周血单个核细胞基因组为模板进行套式PCR扩增HIV1env基因C2 V3区片段 ,将此扩增产物插入T Vector,酶切鉴定重组质粒 ,使用ABI737自动DNA序列测定仪测定序列并用DNASIS软件进行分析。结果　DNA序列资料显示该毒株属于HIV1B亚型衍生株。但与HIV1B亚型的标准株如SF2株相比 ,该HIV1毒株的env基因V3区下游有 192bp的重复插入突变 ,使得该毒株的膜蛋白PND编码基因呈现双V3区的变异 ,该段DNA序列已登录于GenBank(AF2 2 0 2 45 )。结论　该分离株是Objective To sequence and analyze the env gene C2 V3 region of proviral genome from a HIV1 isolate(WWBH7) which was obtained from Huadong Area in China.Methods The env gene C2 V3 DNA fragment was amplified by nested primer PCR with genome DNA of peripheral blood mononuclear cells from a confirmed HIV1 infected individual as a template. The amplified DNA fragment was inserted into pGEM T vector. The recombinant plasmid was confirmed by restriction enzyme analysis. The inserted DNA fragment was sequenced by ABI737 autosequencer and analyzed by PROSIS software.Results The HIV1 strain was the derivatives of HIV1 B subtype. But there was mutation of 192 bp fragment repeated insertion at env C2 V3 region of the HIV1 strain compared with standard HIV1 B subtype such as SF2 strain. The mutation brought about a double V3 region in gene encoded PND (principal neutralizing domains). The DNA sequence was registered in GenBank (AF220245).Conclusion This was a natural mutated variant strain of HIV1 whose genome showed a 192 bp repeated insertion at C2 V3 region of env gene encoded PND of membrane protein.

关 键 词：HIV1变异株 自然突变 env基因C2-V3区 DNA序列 

分 类 号：R373[医药卫生—病原生物学]