小柴胡汤分解剂抑制CVB3m作用及对细胞活性影响  

The inhibitory effect of decomposed Chinese traditional medicine Chaihu on Coxsackie B virus(CVB3m)replication and its influence on cell activity

作　　者：王雪峰[1] 王永梅[2] 刘芳[1] 魏克伦[3] 

机构地区：[1]辽宁中医学院附属医院,沈阳110032 [2]南京中医药大学 [3]中国医科大学第二临床学院儿科

出　　处：《中华实验和临床病毒学杂志》2001年第3期280-282,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金 (3 96 70 86 9);卫生部优秀青年科学基金

摘　　要：目的　探讨小柴胡汤 (XCT)分解剂在体外抗CoxB3病毒 (CVB3m)及保护细胞的作用。方法　采用微量细胞培养技术和中性红摄入法 ,以细胞病变及细胞活性为判定药物细胞毒性及病毒增殖的指标 ,观察不同浓度的XCT及分解 1、2号抗CVB3m的作用及对细胞活性的影响。结果　低于最大无毒剂量浓度的XCT和分解 1号、2号对细胞活性未见明显影响 ,并在一定浓度范围内有促细胞生长、增强细胞活性的作用。治疗组 ,XCT及其分解 1、2号对CVB3m增殖均有明显抑制作用 ,尤以分解 1号作用最明显 ,抑制率为 10 7 6 % ;同等浓度下 ,预防治疗组药物抑病毒率明显高于治疗组 ,最高可达 12 8 1% ;抑病毒吸附组也有明显抑病毒作用。结论　通过比较XCT及其分解 1、2号作用细胞与病毒的不同环节 ,提示XCT具有保护细胞减轻病毒感染功能及直接杀伤CVB3m的作用。Objective To study the anti\|Coxsackie B virus (CVB3m) action of Chaihu(XCT) and its decomposed herb soups No.1 and No.2 \%in vitro\%,and also their protective effect on cells.Methods The anti CVB3m and cell protection effects of XCT and its decomposed herb soups No.1 and No.2 were observed by the mothods of micro cell culture and neutral red ingestion,taking cytopathic effect and cell activity as judgements of medicine toxicity and virus replication.Results The non\|toxic concentrations of XCT and its decomposed herb soups No.1 and No.2 had no apparent influence on HeLa cell activity,on the contrary,in certain range of concentrations,they could promote cell growth and cell activity.In therapeutic cell group,XCT and its decomposed herb soups No.1 and No.2 all had apparent inhibitory effect on CVB3m replication,especially the decomposed No.1 showing an inhibitory rate of 107.6%.Under the same decomposed No.1 concentration(1.5 mg/ml),the viral inhibitory rate of the preventive therapeutic cell group was much more higher than that of the therapeutic cell group,reaching as high as 128.1%.In virus adsorbed cell group,the CVB3m was also obviously inhibited by the XCT and decomposed No.1 and No.2.Conclusion By comparing the effects on cell protection and virus replication of XCT and its decomposed herb soups No.1 and No.2,it identifies that XCT can protect cells against virus infection and directly kill the CVB3m,this Chinese herb medicine may be applied clinically for preventing and curing of viral myocarditis.

关 键 词：柯萨奇病毒B组 细胞活性 小柴胡汤 中药 药理 

分 类 号：R285.5[医药卫生—中药学]