五灵丸与五灵胶囊对免疫性肝损伤小鼠作用的研究  

Study on Effects of Wuling Pills and Wuling Capsules on Immunological Liver Injury in Mice

作　　者：江艳艳[1] 王胜春[1] 刘明义[1] 田卫斌[1] 

机构地区：[1]第四军医大学西京医院,陕西西安710032

出　　处：《中成药》2001年第7期508-510,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :研究并比较五灵丸与五灵胶囊对大鼠肝纤维化的作用。方法 :采用四氯化碳 (CCl4 )致大鼠肝纤维化 ,化学法测定血清羟脯氨酸 (HYP)含量 ,放免法测定血清与肝匀浆Ⅰ型前胶原 (PCⅠ )和Ⅲ型前胶原 (PCⅢ )水平 ,免疫组化法检测肝组织肿瘤坏死因子 (TNF)、纤连蛋白 (FN)的表达。免疫组化方法检测肝组织中TNF和FN ,HE染色观察肝组织细胞形态。结果 :五灵丸与五灵胶囊均能显著降低肝纤维化大鼠血清PCⅠ、PCⅢ和肝匀浆HYP、PCⅠ。五灵胶囊组大鼠肝组FN表达量明显少于模型组。五灵丸组与五灵胶囊组小鼠肝组织内TNF与FN物质呈强阳性。枯否细胞增生肥大 ,内皮细胞被激活。结论 :五灵胶囊确有抗肝纤维化作用。五灵丸与五灵胶囊均能稳定肝细胞膜。Objective: To investigate effects of Wuling Pills and Capsules on immunological liver injury in mice. Methods: The rat liver fibrosis model was induced by carbon tetrachloride. The hydroxyproline (HYP) level in serum was measured by chemical method. The precollagen Ⅰ(PCⅠ) and Ⅲ(PCⅢ) levels in serum and hepatic homogenate were measured by radioimmunoassay. The expressions of tumor necrosis factor (TNF) and fibronectin (FN) in liver tissue were detected by histochemistry method. Moreover, the hepatic histomrphology was observed by HE staining. Results: Both of Wuling Pills and Wuling Capsules could greatly decrease PCⅠ, PCⅢ and HYP levels. The FN expression of liver tissue in Wuling Capsule group was obviously lower than that of the model group. The TNF and FN in liver tissues of rats in the Wuling Pill group and Capsule group showed strong positive. Proliferative kupffer cells and activated endothelial cells were also observed in those two groups. Conclusion: Wuling Capsules has the effect of anti liver fibrosis. Both of Wuling Pills and Capsules can stabilize hepatocyte membrane, and prevent liver cells from the effect of TNF and FN.

关 键 词：肝纤维化 肿瘤坏死因子 纤连蛋白 前胶原 五灵丸 五灵胶囊 

分 类 号：R286.55[医药卫生—中药学] R575.2[医药卫生—中医学]