胶束电动毛细管色谱法分离定量T-2毒素代谢物  

Separation and quantitation of T-2 toxin metabolites by micellar electrokinetic capillary chromatography

作　　者：唐晓波[1] 李德安[1] 冯杰[1] 杨建伯[1] 

机构地区：[1]哈尔滨医科大学大骨节病研究所,黑龙江哈尔滨150001

出　　处：《中国地方病学杂志》2001年第5期378-380,共3页Chinese Jouranl of Endemiology

摘　　要：目的　建立一种测定 T- 2毒素代谢物 T- 2四醇 (T- 2 - 4- ol)、3′-羟基 - HT- 2毒素 (3′- OH- HT- 2 )、3′-羟基 - T- 2毒素 (3′- OH- T- 2 )、T- 2四醇四醋酸 (T- 2 - 4- ol- 4- ac)、T- 2三醇 (T- 2 - 3- ol)、HT- 2毒素和 T- 2毒素的方法。方法　采用胶束电动毛细管色谱法 (MECC)。结果　研究了不同浓度的 SDS和硼砂、不同体积分数的乙腈及不同温度对灵敏度和分离度的影响。发现柱温 30℃ ,所用缓冲液中 SDS的浓度为 6 0 m mol/ L,硼砂浓度为 10mm ol/ L,乙睛的体积分数为 15 %时 ,灵敏度和分离度最高。气压进样 10 s,信号噪音比为 3:1时 ,7种 T- 2毒素代谢物的检测极限 3′- oh- ht- 2是 4.7m g/ L 其余全部为 9.4mg/ L。在上诉条件下 ,所有分析物的浓度与峰面积之间均具有良好的线形关系。结论　 MECC可以用来分离定量 7种 T- 2毒素代谢物 ,为测定人或动物体液中的 T-Objective To develop a method for determination of T-2 toxin metabolites T-2-4-ol, 3′-OH-HT-2, 3′-OH-T-2, T-2-4-ol-4-ac, T-2-3-ol, HT-2, and T-2 toxins.Methods Micellar electrokinetic capillary chromatography (MECC) was used.Results Different parameters concerning the detection limits and resolution such as the concentrations of SDS and borate, varieties of acetonitrile volume and temperature, were studied and optimized. It gave the best results when the column temperature was set to 30℃, the concentration of SDS was 60 mmol/L and borate 10 mmol/L, and is therefore recommended. The limits of detection of the studied T-2 toxin metabolites at a signal-to-noise of 3:1, with an injection time of 10 seconds were 9.4 mg/L for all the studied metabolites except 3′-oh-ht-2(4.7 mg/L). The calibration curve was linear between 9.4 and 150 mg/L for the seven metabolites assayed.Conclusions MECC might be used to detect T-2 toxin metabolites.

关 键 词：胶束电动毛细管色谱法 T-2毒素代谢物 定量 MECC 流行病学 大骨节病 

分 类 号：R684.1[医药卫生—骨科学]