全反式维甲酸可增强大鼠系膜细胞uPA和MMP-2表达  

All-trans Retinoic Acid Upregulates the Expression of uPA/MMP-2 in Cultured Rat Mesangial Cells

作　　者：张农[1] 刘琛[1] 林文生[1] 郭慕依[1] 

机构地区：[1]复旦大学基础医学院病理学教研室,上海200032

出　　处：《复旦学报（医学版）》2001年第4期281-283,共3页Fudan University Journal of Medical Sciences

基　　金：上海市科技发展基金 (98QB14 0 0 7);卫生部优秀青年科技人才专项基金项目;CMB基金资助

摘　　要：目的　研究全反式维甲酸对体外培养大鼠系膜细胞uPA和MMP 2表达的影响 ,探讨全反式维甲酸抗肾小球硬化的机制。方法　分别应用Northernblot、Westernblot和酶谱法检测全反式维甲酸对培养大鼠系膜细胞uPA和MMP 2表达的影响。结果　ATRA可促进系膜细胞uPA和MMP 2蛋白表达 ,提高其酶活性 ,并增强MMP 2mRNA表达。结论　ATRA可增强大鼠系膜细胞的uPA和MMP - 2表达 。Purpose: To study the effects of all-trans retinoic acid on the expression of uPA/MMP-2 in cultured rat mesangial cells, and to elucidate the mechanism by which all-trans retinoic acid prevents age-related progressive glomemular sclerosis. Methods: The mRNA, protein and enzymatic activity of uPA/MMP-2 were detected by Northern blot analysis, Western blot analysis and Zymography assay, respectively. Results: All-trans retinoic acid upregulated the expression of uPA/MMP-2 protein, enhanced the enzymatic activity of uPA/MMP-2. Thus all-trans retinoic acid elevated the level of MMP-2 mRNA in cultured mesangial cells. Conclusions: All-trans retinoic acid upregulated the expression of uPA/MMP-2 in cultured rat mesangial cells. It is valuable for explaining the role of all-trans retinoic acid in preventing age-related glomerulsclerosis.

关 键 词：全反式维甲酸 系膜细胞 尿激酶型纤溶酶原激活物 基质金属蛋白酶-2 ATRA MsC 

分 类 号：R692.6[医药卫生—泌尿科学] Q343.3[医药卫生—外科学]