同时使用三种细胞对急性弛缓性麻痹病例病原学监测结果的影响探讨  

Simultaneous Use of 3 Cell Types for Virological Surveillance of Acute Flaccid Paralysis Cases

作　　者：李爱英[1] 常一华[1] 赵习芳[1] 那丹宇[1] 郑蕾[1] 

机构地区：[1]山西省卫生防疫站,太原030012

出　　处：《中国计划免疫》2001年第4期193-194,共2页Chinese Journal of Vaccines and Immunization

摘　　要：:山西省卫生防疫站脊髓灰质炎 (脊灰 )实验室从 1998年 7月正式使用L2 0B细胞 ,并同时使用RD和Hep - 2细胞进行急性弛缓性麻痹 (AFP)病例病原学监测 ,为了解同时使用 3种细胞对AFP病例病原学监测结果的影响 ,将 3 0 8例AFP病例粪便标本在 3种细胞上的分离状况进行比较分析。同时使用 3种细胞共分离到脊灰病毒 (PV)2 0份 ,其中RD细胞分离到 14份 ,L2 0B细胞 16份 ,Hep - 2细胞 8份 ,总分离率为 6 5 %。分离到非脊灰肠道病毒(NPEV) 65株 ,其中RD细胞 3 0株 ,Hep - 2细胞 4 8株 ,分离率为 2 1 1%。结果显示 ,同时使用 3种细胞对提高PV检测灵敏度和NPEV分离率 ,保证脊灰实验室监测质量有重要意义。The L20B cell was formally used in our polio laboratory since July 1998,RD and Hep-2 cells were simultaneously used for acute flaccid paralysis(AFP)cases surveillance 308 samples of AFP cases were inoculated onto 3 types of cells simultaneously The results of virus isolation were compared and analyzed 20 polioviruses in total were isolated,the positive rate was 6 5%,of which 14,16 and 8 were isolated in RD,L20B and Hep-2 cell respectively 65 non-polio enteroviruses(NPEV)were isolated from AFP cases,30 in RD and 48 in Hep-2,the positive rate was 21 1%. Using 3 kinds of cells simutaneously are very necessary for enhancing the sensitivity of AFP virological surveillance,the isolation rate of NPEV,and to ensure the surveillance quality of poliovirus laboratory

关 键 词：脊髓灰质炎病毒 非脊髓灰质炎肠道病毒 细胞 AFP PV 

分 类 号：R373.22[医药卫生—病原生物学] R744.8[医药卫生—基础医学]