双价痢疾菌苗角结膜免疫豚鼠后泪液中特异性抗体的检测  

The detection of specific antibodies in tears of guinea pigs after immunized with two bivalent Shigella vaccine strains(Ipa^+/Ipa^-)

作　　者：石辛甫[1] 邢丽[1] 陈志华[1] 彭虹[1] 高杰英[1] 

机构地区：[1]军事医学科学院微生物流行病研究所免疫室,北京100850

出　　处：《中国免疫学杂志》2001年第6期302-304,共3页Chinese Journal of Immunology

基　　金：国家"863"基金资助项目!( 863 10 2 0 7 0 3 0 3 );973项目!(G19990 5 41 0 3 )

摘　　要：目的 :研究痢疾杆菌侵袭蛋白与局部免疫反应的关系 ,为痢疾菌苗的研制提供理论依据。方法 :用表达和不表达侵袭蛋白 (Ipa+ /Ipa-)的两株福氏、宋内氏双价痢疾菌苗经角结膜免疫豚鼠 ,并分别用福氏、宋内氏毒株攻击 ,BA ELISA方法检测豚鼠泪液中特异性抗体升高情况。结果 :发现角结膜免疫后豚鼠泪液中特异性双价抗体水平较对照显著升高 ,差别具有统计学意义 ,Ipa+ 菌苗免疫组宋内氏抗体水平较Ipa-免疫组升高 ,差别具有统计学意义 ;攻击后 ,两免疫组局部抗体呈二次反应变化。结论 :侵袭蛋白的表达可加强痢疾菌苗诱导的局部抗体水平。两株双价痢疾菌苗均有较好的免疫原性。Objective:To detect the influence of Ipas, and construct better Shigella vaccines.Methods: Guniea pigs were immunized with Ipa + and Ipa - bivalent Shigella vaccines strains by eyes mucosal immunization, and challenged with willed strains. The specific antibodies in tears were measured by BA ELISA assays after immunization and challenge. Results: The levels of specific local specific antibodies of immunized groups increased significantly compared to the control group and Ipa + group compared to Ipa - group after immunization.Conclusion: The expression of invasive plasmid antigens(Ipa) can enhance the levels of specific local antibodies. The two Shigella vaccines have good immunogenicity.

关 键 词：侵袭蛋白 双价痢疾菌苗 特异性抗体 免疫生物学 局部抗体水平 免疫原法 

分 类 号：R392.1[医药卫生—免疫学]