真菌对人参皂苷Rb_1及人参二醇系皂苷的代谢作用  

METABOLISM OF GINSENOSIDE Rb_1 AND PANAXADIOL SAPONINS BY FUNGI

作　　者：马吉胜[1] 周秋丽[2] 费晓方[3] 孙晔[1] 王本祥[1] 

机构地区：[1]长春中医学院新药研究中心,吉林长春130021 [2]白求恩医科大学生物工程研究所,吉林长春130021 [3]吉林大学生命科学院,吉林长春130023

出　　处：《药学学报》2001年第8期603-605,共3页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助课题 (395 70 86 1)

摘　　要：目的　研究真菌对人参皂苷Rb1 (G Rb1 )及人参二醇系皂苷 (PDS)的代谢作用。方法　在恒温振荡条件下 ,10种真菌对其底物G Rb1 及PDS分别进行代谢 ,不同时间采集代谢物 ,正丁醇萃取 ,用薄层色谱 (TLC)、高效液相色谱 (HPLC)和电喷雾质谱 (ESI MS)检测代谢成分。结果　代谢产物中存在化合物G Rb1 ,G Rd ,G F2 ,CK和Ppd。结论　发现 6种真菌对底物有不同程度的代谢作用 ,其代谢过程可能为G Rb1 (或PDS)→G Rd→G F2 →CK→Ppd ,并通过控制这一过程可获得目的代谢产物CK。AIM To study the metabolic process of ginsenoside Rb 1 (G Rb 1) and panaxadiol saponins (PDS) by fungi. METHODS Ten strains of fungi were incubated with G Rb 1 and PDS at a certain temperature with shaking. A portion was taken out at different time and mixed up with butanol. The butanol extract was analysed by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and electrospray ionazition mass spectrometry (ESI MS). RESULTS It was found that there were ginsenoside Rd (G Rd), ginsenoside F 2 (G F 2), compound K (CK) and 20( S ) protopanaxadiol (Ppd) metabolites beside the prodrug G Rb 1 induced by fungi (No.1,2,3,6,8,9). CONCLUSION The six strains of fungi have different degrees of ability to metabolize G Rb 1 and PDS. The possible metabolic process could be as follows: G Rb 1 (or PDS)→G Rd→G F 2→CK→Ppd.

关 键 词：真菌 人参皂苷RB1 人参二醇系皂苷 代谢 

分 类 号：R969.1[医药卫生—药理学] R285.5[医药卫生—药学]