短间隔连续部分肝切除(SISPH)对大鼠肝ACP、AKP、HSC70/HSP68和PCNA的影响  

EFFECTS OF SHORT INTERVAL SUCCESSIVE PARTIAL HEPATECTOMY ON ACP.AKP.HSC70/HSP68 AND PCNA IN RAT LIVER

作　　者：徐存拴 卢爱灵[1] 封青川[1] 李庚午[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《实验生物学报》2001年第3期197-206,共10页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(39970362);河南省生物工程重点实验室资助项目(PKL98001)

摘　　要：本文以间隔时间(4h和36h)相互交叉的短间隔连续部分肝切除(36-4-4 SISPH和4-36-36-36SISPH)为模型,分析了ACP、AKP、HSC70/HSP68和PCNA的活性和含量变化。结果表明:140 kD的ACP和AKP活性在两种模型中都随着肝切除次数的增加而增加,而160 kD的AKP和ACP则与此相反;PCNA在4-36-36-36 SISPH中比在36-4-4SISPH中表达更多,而HSC70/HSP68的表达则与此相反。可见,连续部分肝切除的次数和方式能影响ACP、AKP、HSC70/HSP70和PCNA的活性和含量,并讨论了其可能的机理和生理意义。In this paper,the models of 36-4-4 SISPH and 4-36-36-36 SISPH were used lo analyze the changes of activity and content of ACP, AKP, HSC70/HSP68 and PCNA in rat liver. The results showed that the activities of 140 kD ACP and AKP in SISPH were increased following the increase of SISPH number of times, but that of 160 kD ACP and AKP were decreased following the increase of SISPH number of times. The content of PCNA in 4-36-36-36 SISPH were more than that in 36-4-4 SISPH, in contrast for HSC70/HSP68 in these two models. Therefore, the content and activities of ACP, AKP, HSC70/HSP68 and PCNA could be strongly effected by SISPH number of times and SISPH methods. Its mechanisms and physiological significance were discussed.

关 键 词：短间隔连续部分肝切除 大鼠肝 ACP AKP 热休克蛋白 PCNA 肝再生 

分 类 号：Q819[生物学—生物工程]