应用荧光原位杂交技术检测人β~E珠蛋白基因在转基因小鼠染色体上的整合状态  

Localization of Human β~E-globin Gene in Chromosomes of Transgenic Mice by Fluorescence in situ Hybridization

作　　者：夏薇[1] 刘德培 董文吉 李斌[1] 郭志晨 王晶 李铁昌 梁植权 

机构地区：[1]中国医学科学院基础医学研究医学所中国协和医科大学基础医学院医学分子生物学国家重点实验室,北京100005

出　　处：《遗传》2001年第5期397-400,共4页Hereditas（Beijing)

基　　金：国家高技术发展计划 ( 86 3)资助 (Z2 0 -0 2 -0 2 )

摘　　要：为将荧光原位杂交技术应用于基因定位研究中 ,探讨一种能有效地检测转基因动物染色体上外源基因整合状态的实验方法 ,对小鼠腹腔注射秋水仙素后 ,取转基因小鼠骨髓制备中期染色体 ,将传统的FISH方法加以改进 ,检测外源基因在转基因小鼠染色体上的整合状态。检测结果表明 ,外源人 βE 珠蛋白基因已稳定地整合于小鼠染色体上。FISH能直观地反映外源基因在转基因动物染色体上的整合状态 ,该方法可对转基因动物及基因转移研究中的外源基因整合后进行染色体定位检测。To determine the integration site of human β E globin gene in the chromosomes of transgenic mice,transgenic mice carrying human β E globin gene were injected intraperitoneally with colchicines,then,bone marrow cells were isolated and metaphase chromosomes were prepared, the traditional FISH method was improved to detect the integration site of human β E globin gene in transgenic mice when combined with G banding.Human β E globin gene can be detected in different position of different chromosomes in transgenic mice and FISH signals showed that two mice were heterozygous of human β E globin gene and one was homozygous.Human β E globin gene was integrated into the chromosomes of transgenic mice in a random pattern and the results demonstrated that FISH can be used to investigate the integration site of foreign genes in transgenic mice.

关 键 词：荧光原位杂交技术 人β^E珠蛋白基因 转基因小鼠 整合位点 染色体 

分 类 号：S865.131[农业科学—野生动物驯养]