Bt水稻“克螟稻”花粉对家蚕生长发育的影响  

The Effect on the Development of Silkworm Larvae of Transgenic Rice Pollen with a Synthetic cry1Ab Gene from Bacillus thuringiensis

作　　者：王忠华[1] 倪新强[2] 徐孟奎[3] 舒庆尧[1] 夏英武[1] 

机构地区：[1]浙江大学原子核农业科学研究所,杭州310029 [2]浙江大学实验设备处,杭州310031 [3]浙江大学蚕学系,杭州310029

出　　处：《遗传》2001年第5期463-466,共4页Hereditas（Beijing)

基　　金：受国家植物转基因专项 (J2 0 0 0$CB$C 0 0 9) ;浙江省科委重大项目 ( 99110 2 0 0 4$C 0 0 2 );美国洛氏基金 ( 2 0 0 0FS0 2 0 )共同资助

摘　　要：本试验以家蚕为供试对象研究Bt水稻“克螟稻”花粉对家蚕生长发育的影响。结果发现 ,与正常无花粉处理相比 ,无论是非Bt水稻花粉处理 ,还是Bt水稻花粉处理 ,对初孵家蚕幼虫的致死率无多大影响 ,而对家蚕的体重有较大影响 ,其中三龄期家蚕体重存在极显著差异。还发现Bt水稻花粉处理组家蚕在 3龄时期大小很不一致 ,最轻体重为 18.1mg ,而最重体重为 183 .8mg。这是由于采用人工抖粉桑叶上花粉浓度不均匀造成的。鉴于实际生态条件下桑叶上的花粉浓度可能远远低于试验条件 ,因此 ,在实际稻桑共作环境下 ,Bt水稻“克螟稻”花粉对家蚕生长发育可能不会造成太大的影响。The effect on the development of silkworm larvae of Bt transgenic rice pollen containing cry1 Ab gene from Bacillus thuringiensis were investigated.Compared with normal treatment,mortality of newly hatched silkworm larvae in either Bt rice pollen or susceptible rice pollen treatment were not significantly different,while the variances of silkworm larvae weight at third instar were significant at 0.01level.In addition,the weight of each silkworm larva at third instar in Bt rice pollen treatment showed a big difference,the biggest and smallest silkworm larvae were 183.8mg and 18.1mg respectively,which was probably caused by the difference of Bt rice pollen concentration on the mulberry leaves.As pollen concentration on the mulberry leaves in actual field was lower than in lab,the influence on the development of newly hatched silkworm larvae of Bt rice pollen is not likely significant in actual ecological environment.

关 键 词：Bt转基因水稻 花粉 家蚕 生长发育 生态风险 稻桑共作 

分 类 号：S884.9[农业科学—特种经济动物饲养]