人工反义基因对HLF细胞α1(Ⅰ)胶原基因表达的抑制作用  

Inhibition of α1（Ⅰ）collagen gene expression in human lung fibroblasts by artificial antisense gene

作　　者：单越新[1] 罗超权[2] 

机构地区：[1]第一军医大学生物化学教研室,广东广州510515 [2]中山医科大学生物化学教研室,广东广州510080

出　　处：《第一军医大学学报》2001年第8期596-598,共3页Journal of First Military Medical University

摘　　要：目的 研究纤维增生性疾病与Ⅰ型胶原蛋白异常表达的关系。方法 通过构建人工反义α1（Ⅰ）胶原基因真核表达质粒，探索反义 RNA对人胚肺成纤维细胞（HLF 细胞）α1（Ⅰ）胶原基因表达的抑制作用。结果 含有第Ⅰ内含子与第Ⅲ外显子区域的反义α1（Ⅰ）胶原基因片段的真核表达质粒能够在转录和翻译两个水平抑制α（Ⅰ）胶原基因的表达。其中mRNA水平的抑制率为56.84%； 蛋白水平的抑制率为54.24%。结论 反义RNA能够在转录和翻译两个水平抑制HLF细胞α1（Ⅰ）胶原基因的表达。Objective To investigate the correlation of hypertrophic diseases with the over-expression of typeⅠcollagen, and provide experimental basis for gene therapy of the hypertrophic disease. Method Eukaryotic recombinant plasmids containing antisense α1（Ⅰ） collagen gene were constructed to study how antisense nucleic acids regulate the expression of α1Ⅰ）collagen gene in human lung fibroblasts (HLFs). Result The eukaryotic expression plasmids of α1（Ⅰ）collagen inhibited the transcription of the target gene at a rate up to 56.8% (at mRNA level) and type Ⅰpre-collagen expression in the transfected HLFs by 54.24% (at the protein level ). Conclusion The expression of α1（Ⅰ） collagen gene in HLFs can be inhibited through both transcription and translation by antisense RNA.

关 键 词：α1(Ⅰ)胶原基因 反义核酸 HLF细胞 人工反义基因 纤维增生性疾病 

分 类 号：Q78[生物学—分子生物学]