受体针对供体抗原特异性T淋巴细胞克隆的建立及其形成条件的比较研究  

THE COMPARATIVE STUDY ON THE ESTABLISHMENT AND ITS CONDITION FOR THE CLONE OF RECIPIENT AGAINST DONOR ANTIGEN-SPECIFIC T LYMPHOCYTES

作　　者：周军[1] 王磊[1] 兰平[1] 汪建平[1] 

机构地区：[1]中山医科大学附属第一医院胃肠外科,广州510080

出　　处：《中国普外基础与临床杂志》2001年第5期292-294,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：广东省自然科学基金 (项目编号 :970 0 5 5 );广东省科委博士基金 (项目编号 :9842 2 9)资助研究

摘　　要：目的　探索建立受体针对供体抗原特异性T淋巴细胞克隆 ,转入自杀基因诱导移植耐受的可行性。方法　供体鼠脾细胞免疫受体鼠 ,再分离、纯化经供体鼠抗原刺激受体鼠T淋巴细胞 ,将其作有限稀释为单个T淋巴细胞 ,再分别以不同浓度饲养细胞、ConA、IL 2等条件 ,促使T淋巴细胞分裂、增殖 ,构建受体针对供体抗原特异性T淋巴细胞克隆。结果　①受体针对供体抗原特异性T淋巴细胞克隆稳定建立 ;②不同免疫组间克隆生成率的差异有显著性意义 (t>t0 .0 5 ) ;无或单纯饲养细胞孔无克隆生长 ;ConA刺激有少数克隆生成 ;克隆生成率与IL 2刺激浓度可能相关。结论　成熟T淋巴细胞 ,在一定条件下仍可发生分裂和增殖 ,形成克隆。通过供体脾细胞抗原特异性刺激 ,提示最终筛选出的T淋巴细胞克隆具有针对供体抗原的免疫反应性。Objective To explore the feasibility that the recipient against donor antigen specific T lymphocytes clones are formed,and the suicide genes are induced into the clone. In the end it may induce the transplanted organ tolerance. Methods The recipient rats were immunized by the donor rats splenocytes, then the recipients T cell were isolated, purified and diluted with limited dilute methods into single cell.The T cells were cultured by adding raise cells,ConA or IL 2 under the different concentration and at last the T cell clone were formed. Results A stable recipient against donor antigen specific T cell clone was established. The difference among the different immune groups was very significant ( t > t 0.05 ). The T cell clone was not formed without raise cells or only with raise cells. The rare clone could be formed by ConA stimulation but without IL 2. The clone forming rate was associated with concentration of IL 2. Conclusion The mature T cell is able to proliferate to form clone when the condition is suitable. The recipient is immunized with donor rats spleen cellular antigen. The T cells clones selected in the end are donor antigen specific.

关 键 词：供体抗原特异性 T淋巴细胞 克隆 有限稀释法 饲养细胞 移植免疫耐受 

分 类 号：R392.4[医药卫生—免疫学]