rhTNFα加rhIL-2诱导小鼠CNE3细胞凋亡和抑制增殖的体内实验研究  

Synergistic Antitumor Effect of Recombinant Human Tumor Necrosis Factor-α and Interleukin-2 in Nude Mice Bearing Nasopharyngeal Carcinoma

作　　者：焦伟[1] 刘时才[1] 莫祥兰[1] 黄振录[1] 

机构地区：[1]广西壮族自治区人民医院,南宁市530021

出　　处：《中国肿瘤临床》2001年第7期549-551,共3页Chinese Journal of Clinical Oncology

基　　金：广西壮族自治区科学研究与技术开发计划项目资助(桂科基9631013)

摘　　要：目的：研究重组人肿瘤坏死因子（rhTNF－α）对鼻咽癌细胞敏感性、重组人白细胞介素－2（rhIL－2）协同抗瘤效应及抗瘤机理。方法：利用我室建立的鼻咽癌细胞株（CNE3）制成的裸鼠鼻咽癌模型，rhTNFα通过与rhIL－2配伍瘤内注射以观察移植瘤超微结构及免疫组织化学的改变，探讨其作用机理。结果：鼻咽癌裸鼠移植瘤经治疗后出现坏死、体积缩小、甚至消失；超微结构观察凋亡细胞缩皱，核内异染色质边集；免疫组化研究发现：rhTNF－α有下调（突变型）p53和增殖细胞核抗原（PCNA）蛋白表达，其可能与rhTNF－α的抗癌机理有关；rhIL－2能增强rhTNF－α的抗瘤作用。结论：rhIL－2增强rhTNF－α的抗瘤机理可能与IL－2诱导癌细胞表达TNF受体作用有关，该研究为临床在鼻咽镜下进行TNF局部治疗提供实验依据。Objective To study the synergistic effect of recombinant human tumor necrosis factor rhTNF－α combined with recombinant human interleukin－2 rhIL－2 on nasopharyngeal carcinoma NPC． Methods Nude mice model bearing NPC were established with human nasopharyngeal carcinoma cell line CNE3 in our laboratory． RhTNF－α and rhIL－2 were administered singly or incombinatively． After 3 or 5 weeks treatment with rhTN or／and rhIL－2 the nude mice were killed and the tumor were harvested． The tumor tissues were stained with immunohistochemical method for p53 and PCNA electron microscopy EM was used also． Results After treatment the tumor cells showed necrosis regression and impaired ultrastructures． There was a notable synergistic antitumor effect by the combination use of rhTNF－α and rhIL－2． Immunohistochemical stain showed that rhTNF－α down－regulated expression of p53 mutation and PCNA protein． Conclusion rhIL－2 appears to enhance the cytotoxic activity of rhTNF－α． These studies provide an experimental basis for biological treatment of NPC．

关 键 词：重组人肿瘤坏死因子α 白细胞介素-2 鼻咽癌细胞株 裸鼠 抑瘤效应 RHTNFΑ RHIL-2 

分 类 号：R730.2[医药卫生—肿瘤]