西双版纳小耳猪DNA指纹分析  

DNA Fingerprinting for Banna Miniature Pig

作　　者：陈丙波[1] 周建华[1] 魏泓[1] 曾养志[2] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]云南农业大学动物遗传资源研究室,昆明650201

出　　处：《遗传》2001年第4期295-297,共3页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目 (G2 0 0 0 0 16 10 6 );国家自然科学基金重点项目 (NO .39830 190 );国家"九五"科技攻关项目 (96

摘　　要：采用ECL核酸直接标记和检测试剂盒 ,以HRP标记JL - 0 2多位点探针 ,对云南版纳小耳猪的HinfⅠ或HaeⅢ酶切图谱进行Southern杂交 ,以化学发光法进行检测 ,获得了清晰可辨的、具有高度多态性的DNA指纹图谱。大于 2kb的谱带数在 13～ 17条之间 ,JB亚系内 80 5、80 7家系和JS亚系内 111、12 1、133、15 1家系不同个体间的相似系数分别为 0 .92± 0 .0 4、0 .85± 0 .13、0 .86± 0 .0 2、0 .80± 0 .0 5、0 .84± 0 .0 9、0 .88± 0 .0 8,显著高于家系间的相似系数。JB亚系内的 80 5家系和JS亚系内的 15 1家系不同个体间的相似系数最大 ,分别为 0 .92和 0 .88,说明其近交程度较高 ,个体间差异较小 ,均一性较强。另外 ,JB亚系内的 80 5与 80 7两个家系不同个体猪在 11.5kb处 ,均有一条区别于其他家系的特有图带 ,其是否是导致JB亚系体型较大的特异性基因座 ,有待进一步研究。Clearly and highly polymorphic DNA fingerprints from Banna miniature pig were obtained by Southern hybridization with a nonisotopically HRP(Horseradish peroxidase) labelled multiloci minisatellite probe JL-02,the fragment number of which were ranged from 13 to 17.The similarity coefficient of various individuals from family “805”、“807”in substrain JB and family“111”、“121”、“133”、“151”in substrain JS was 0 92±0 04、0 85±0 13、0 86±0 02、0 80±0 05、0 84±0 09、0 88±0 08,which is higher than that between families(0.46～0.65).Furtherly,similarity coefficients of family “805” in substrain JB and family “151” in substrain JS were 0.92 and 0.88 respectively.This showed that the two families have the higher inbreeding degree,smaller genetic difference.among individuals and the better homogeneity.In addition,all individuals from family 805、807 in substrain JB have a unique fragment different from other individuals from other families.which is abount 11.5kb long.This suggested that this may be the gene resulted in large bodyweight of substrain JB,further research is necessary to be conducted.

关 键 词：DNA指纹 西双版纳小耳猪 SOUTHERN杂交 

分 类 号：Q959.842[生物学—动物学]