一株纤维素降解菌的鉴定  

Identification of a Cellulose-hydrolytic Bacterium

作　　者：祝令香[1] 徐建[1] 于巍[1] 董志扬[1] 林敏[2] 陆伟[2] 平淑珍[2] 

机构地区：[1]中国科学院微生物研究所,北京100080 [2]中国农业科学院原子能利用研究所,北京100094

出　　处：《农业生物技术学报》2001年第3期255-257,共3页Journal of Agricultural Biotechnology

基　　金：中国科学院创新工程项目;课题号:KSCX2-3-02-03

摘　　要：采用１６ｓｒＤＮＡ序列分析，形态鉴定和Ｂｉｏｌｏｇ　Ｍｉｃｒｏｓｔａｔｉｏｎ　Ｉｄｅｎｔｉｆｉｃａｔｉｏｎ　Ｓｙｓｔｅｍ　对一株强降解纤维素细菌Ｚ２１５进行了鉴定。１６ｓｒＤＮＡ序列分析表明，Ｚ２１５与多株纤维单胞菌的１６ｓｒＤＮＡ　的同源性均在９５％以上，在细菌系统分类学上应归属于纤维单胞菌属（Ｃｅｌｌｕｌｏｍｏｎａｓ）。从构建的系统发育树中可以看出，Ｚ２１５与Ｃ．ｇｅｌｉｄａ，及Ｃ．ｇｅｌｄａ共同构成一个分枝，该菌与Ｃ．ｇｅｌｉｄａ和Ｃ．ｇｅｌｄａ的１６ｓｒＤＮＡ同源性分别为９８．６％和９８．５％。该菌的形态鉴定与凝胶纤维单胞菌（Ｃ．ｇｅｌｉｄａ）比较相近。而该菌的Ｂｉｏｌｏｇ生理鉴定也表明，Ｚ２１５与Ｃ．ｇｅｌｉｄａ的相似性最高，为０．５５５，但存在一些不同；因此初步确定Ｚ２１５为凝胶纤维单胞菌的一个变种（Ｃ．ｇｅｌｉｄａ　ｖａｒ）。s: A strain Z215,which can hydrolyze natural cellulose efficiently, was identified by 16srDNA sequence, morphological identification and Biolog Microstation Identification System. The sequence data was compared with published sequences of relative bacteria from Genbank. The results showed that strain Z215 is belonged to genus Cellulomonas. In the phylogenetic tree, strain Z215,C.gelida and C.gelda constituts a branch;the similarity values of 16srDNA sequence with comparing those strains are 98.6% and 98.5%, respectively.The morphological identification of Z215 showed that it is belonged to the species of C.gelida.The Biolog Microstation Identification System also showed that the best similarity ID is C.gelida(the value=0.555),but the physiological data between them had some difference.So the strain Z215 is considered a new mutant of C.gelida.

关 键 词：16srDNA 生理鉴定 凝胶纤维单胞菌 纤维素 降解能力 

分 类 号：Q939.11[生物学—微生物学]