含四个串联核酶和GFP基因的转基因质粒的构建  

Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baculovirus Gene

作　　者：邹键[1] 沈学仁[1] 吴建华 周国瑛[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《中国病毒学》2001年第3期275-279,共5页Virologica Sinica

基　　金：国家海洋863计划经费资助项目 ( 819 0 4 0 8)

摘　　要：以含绿色荧光蛋白 (GFP)基因的质粒pSK10 0 DS、含切割对虾杆状病毒基因的核酶Rz1的质粒pRGRz1、含核酶Rz2的质粒pRGRz2和转基因空质粒pcDNA3为基础 ,把绿色荧光蛋白GFP基因克隆于pcDNA3的SV40启动了下面 ,由SV40启动子控制 :含四个两种核酸基因的四联体克隆于pcDNA3的多克隆位点区 ,由T7启动子控制 ,构建成含两个Rz1、两个Rz2和GFP基因的转基因质粒pGTR ,以用于转基因抗病毒对虾的研究。The transgenic plasmid of hammerhead ribozymes Rz1 and Rz2 targeting one of the Prawn baculovirus genes has been coustructae.The transgenic plasmid contains double series copies of Rz1 and Rz2 as Rz1-Rz2-Rz1-Rz2.The Green Fluorescent Protein(GFP)gene was selected as the report gene of the transgenic plasmid.The GFP gene was cloned into plasmid pcDNA3 at site 2093bp with Sma I,controlled by SV40 promoter.Ribozyme gene Series was cloned into the MCS of plasmid pcDNA3 with proper restriction endonuclease,controlled by T7 and CMV promoter.The constructed transgenic plasmid was named pGTR and will be used for obtaining transgenic Prawn.

关 键 词：对虾杆状病毒 核酶 对虾转基因质粒 绿色荧光蛋白 

分 类 号：S941.41[农业科学—水产养殖]