丙型肝炎病毒CS,NS3 NS4区抗原融合基因的构建及其在大肠杆菌中的表达  

Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression

作　　者：李铭[1] 沈小川[2] 林矫矫 倪振亚 苑纯秀 刘金明 杨冠珍[2] 蔡幼民 吴祥甫[2] 

机构地区：[1]上海动物生物技术研究中心 [2]中国科学院上海生化研究所,上海200031

出　　处：《中国病毒学》2001年第3期283-285,共3页Virologica Sinica

基　　金：上海市科技兴农重点攻关项目 (农科攻字 2 0 0 0第 5 8号 )

摘　　要：DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a(+)and was expressed in Escherichia coli.The expressed protein was purified by Ni(+)NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay.DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a(+)and was expressed in Escherichia coli.The expressed protein was purified by Ni(+)NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay.

关 键 词：丙型肝炎病毒 抗原融合基因 蛋白表达 免疫印迹试验 CS区 NS3区 NS4区 

分 类 号：R512.63[医药卫生—内科学] R373.21[医药卫生—临床医学]