表达粒细胞巨噬细胞集落刺激因子的非复制痘苗病毒的抗肿瘤效果  

Anti-tumor Immune Effects of GM-CSF Expressed by Non-replicating Vaccinia Virus

作　　者：郭斐[1] 陆柔剑[1] 许洪林[1] 李军[1] 张颖妹[2] 马大龙[2] 阮力[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052 [2]北京医科大学免疫系,北京100083

出　　处：《病毒学报》2001年第3期231-235,共5页Chinese Journal of Virology

基　　金：国家 8 63高技术研究发展计划 (863 -10 2 -0 7-0 2 -0 1);国家自然科学基金 (3 95 2 5 0 0 1) (3 0 0 70 711)

摘　　要：构建了表达GM CSF的非复制型重组痘苗病毒RVΔ11β75GMCSF。Southernblot证实重组病毒C K片段间基因缺失 ,同时插入GM CSF基因 ,GM CSF可以分泌形式良好表达。Wistar大鼠Walker's瘤内注射重组病毒RVΔ11β75GMCSF后 ,可以延长荷瘤鼠的生存时间 ;重组病毒RVΔ11β6 5GMCSF修饰的肿瘤细胞裂解物(WRC2 5 6 )的肿瘤免疫治疗 ,可以抑制荷瘤大鼠的肿瘤生长 ,瘤重较对照组减轻。此结果提示 :非复制重组痘苗病毒表达的GMRecombinant vaccinia virus RVΔ11β75GMCSF which can express GM CSF was constructed by using non replicating vaccinia virus vector pNEOCK11β75GMCSF and vaccinia virus Tian Tan strain.By Southern blot,it was demonstrated that the genes between HindⅢ C and K fragments of vaccinia virus were deleted and GM CSF gene was integrated stably.The integrated GM CSF gene can be exprssed through secreting manner.In the Walker's tumor model of Wistar rats,direct injection of recombinant virus RVΔ11β75GMCSF in tumor tissue resulted in prolonged life span of Wistar rats and also vaccinia virus RVΔ11β75GMCSF modified WRC256 oncolysates reduced tumor hyperplasia.In conclusion,GM CSF may be effectively used as adjuvant in cancer immunotherapy.

关 键 词：非复制重组痘苗病毒 细胞巨噬细胞集落刺激因子 免疫治疗 抗肿瘤作用 

分 类 号：R373.12[医药卫生—病原生物学] R730.51[医药卫生—基础医学]