精制Vero细胞狂犬病疫苗的灭活和纯化  

Research on Procedures of Inactivation and Purification for Purifying Vero Cell Rabies Vaccine

作　　者：吕宏亮[1] 宋继萍[2] 段招军[1] 邹勇[2] 沈心亮[2] 王玉琳[2] 周书全[2] 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室,北京100052 [2]兰州生物制品研究所,兰州730046

出　　处：《病毒学报》2001年第3期236-239,共4页Chinese Journal of Virology

基　　金：科教部"九五"攻关项目 (96-C0 2 -0 3 -0 4)

摘　　要：通过狂犬病病毒灭活和纯化试验 ,试制精制Vero细胞狂犬病疫苗。疫苗经检测残余小牛血清白蛋白含量、Vero细胞残余DNA含量、疫苗效价、安全试验均能达到WHO规程要求。The procedure of inactivation and purification for purifying Vero cell rabies vaccine(PVRV)was explored by studying on inactivation with beta propiolactone(BPL),and purification with chromatography.The residual calf serum contents,residual Vero cell DNA contents,potency and safety tests of the purified Vero cell rabies vaccine prepared with this procedure all met the WHO Requirements of Biological Products(Edition 1986),so this procedure of inactivation and purification was available for large scale production of purified Vero cell rabies vaccine.

关 键 词：VERO细胞狂犬病疫苗 灭活 纯化 

分 类 号：R512.90[医药卫生—内科学] R473.9[医药卫生—临床医学]