马铃薯Y病毒蚜传辅助成分介导PVX/PVY协生作用  

Helper Component Proteinase of Potato Y Potyvirus Mediates Synergism of PVY and Potato X Potexvirus

作　　者：鲁瑞芳[1] 郭明[1] 李为民[1] 梁小波[1] 彭学贤[1] 

机构地区：[1]中国科学院微生物所植物生物技术实验室,北京100080

出　　处：《病毒学报》2001年第3期250-254,共5页Chinese Journal of Virology

基　　金：国家自然基金项目 (No3 980 0 0 0 1)~~

摘　　要：构建了马铃薯Y病毒中国株系 (PVY C)蚜传辅助成分 (HC Pro)基因的正义、反义和缺失三种植物表达载体 ,通过农杆菌介导法转化烟草品种NC89。Southernblot分析表明 ,HC Pro基因及其突变体已经整合到烟草染色体中 ,Westernblot分析证明 ,正义HC Pro基因及其缺失突变体在转基因烟草中有表达产物。攻毒试验结果证明 ,转正义HC Pro基因及其缺失突变体不仅能够提高T1转基因烟草中PVY C的病毒积累和致病性 ,而且对异源病毒PVX具有同样的作用 ,而转反义HC Pro基因烟草对PVY C和PVX的致病性无影响。因此PVY CHCPlant transformation vectors expressing sense,antisense and the 3' truncated form of the helper component proteinase (HC Pro) gene of potato virus Y of Chinese strain(PVY C) were constructed,and transformed into tobacco (Nicrotinum tobaccum cv.NC 89 ) by Agrobacterium mediated transformation.Southern and Western blot analyses showed that PVY C HC Pro gene and its deletion mutant at the 3' terminus were integrated into tobacco chromosomes,and expressed their corresponding proteins in transgenic tobacco plants.The challenge tests indicated that the full length HC Pro gene in sense orientation and its deletion mutant not only increased the accumulation and pathogenicity of PVY C in T 1 transgenic tobacco plants,but also had the same effect on a heterologous virus of potato X potexvirus.However,the full length HC Pro gene in antisense orientation had no any influence on the pathogenicity of both plant viruses.Thus, this result demonstrated that PVY C HC Pro gene could mediate synergism of PVX/PVY.

关 键 词：马铃薯Y病毒 HC-PRO基因 致病性 PVX/PVY 协生作用 蚜传辅助成分 

分 类 号：S432.41[农业科学—植物病理学] S435.32[农业科学—农业昆虫与害虫防治]