药用植物栝楼的组织培养及其表达蛋白的分析  

The Tissue Culture of Medicinal Plant Trichosanthes Kirilowii and its Protein Analysis

作　　者：郑树松[1] 苑华毅[2] 王莉江[2] 安成才[2] 陈章良[2] 

机构地区：[1]云南省农业科学院油料作物研究所,昆明650205 [2]北京大学生命科学学院

出　　处：《生物工程学报》2001年第4期420-422,T001,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助! (3 9980 0 0 3 )&&

摘　　要：对栝楼的快速繁殖、愈伤组织的诱导与再分化 ,以及不同培养体系中天花粉蛋白的表达进行了初步研究。结果表明 :栝楼茎切段的腋芽和顶芽在MS +0 5、1 0mg L 6 BA培养基上可以快速繁殖 ;组织培养苗的叶片切块在MS +4 0mg L 6 BA +0 2mg LIAA的培养基上可形成愈伤组织 ,该愈伤组织在 30d后再分化为绿苗 ,绿苗分化率为0 2 5苗 外植体 ;绿苗转移至MS +0 1mg LNAA的培养基可 10 0 %生根 ;生根苗移栽至土壤中 10 0 %成活 ;移栽成活的栝楼在 30d后长出小块根 ,并检测到天花粉蛋白的表达。We reported preliminary results of rapid propagation,callus induction and regeneration of \%Trichosanthes Kirilowii\%,and its protein analysis.Pre-existing meristerms regenerate shoots very rapidly when grown on MS medium containing 0 5 or 1 0mg/L 6-BA;calli could be induced from leaf sections when put on MS medium containing 4 0mg/L 6-BA +0 2mg/L IAA;shoots regenerated successfully 30 days after calli induction and the differentiation ratio was one shoot out of every four leaf sections;and all shoots gave rise to roots after removing onto MS medium containing 0 1mg/L NAA and 100% survived when transplanted into soil.Very excitingly,these plants produced small tubers in one month,where satisfactory expression of TCS protein was detected by Western blot analysis.

关 键 词：栝楼 组织培养 天花粉蛋白 药用植物 蛋白质分析 

分 类 号：Q946[生物学—植物学]