用于寡核苷酸芯片制备载体的醛基载玻片的制备方法优化及性质研究  

Preparation Optimization and Properties of the Aldehyde Microscopic Slides for Oligonucleotide Microarray Fabrication

作　　者：邹宗亮[1] 王升启[1] 王志清[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《生物工程学报》2001年第5期498-502,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金重点项目资助 (398890 0 1)&&

摘　　要：优化了醛基载玻片的制备方法 ,探讨了醛基修饰载玻片固定寡核苷酸探针的性质。研究发现氨基硅烷试剂的浓度是影响载玻片荧光背景的主要因素 ;2 %氨基化试剂处理 16min、戊二醛处理 30min可以得到荧光背景较低、固定效果较好的醛基载玻片。寡核苷酸固定过程中 ,末端氨基修饰没有明显的特异性 ,但是可以提高被固定探针的杂交容量。在较低的浓度 (小于 10 μmol L)时 ,探针的浓度与杂交信号趋近线性关系 ,浓度为 2 0 μmolThe process for preparing the aldehyde slides was optimized and the properties of the aldehyde microscopic slides for immobilizing oligonucleotide were explored. The result shows that the concentration of aminosliane reagent plays an important role in the fluorescent background. Aldehyde slides with 2% aminosilane and 5% aledehyde treatment for 16 min and 30 min respectively immobilize oligonucleotide efficiently and have low fluorescence background. During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides. At low concentration (less than 10 μmol/L), hybridization signal has linear relationship with probe concentration, the hybridization signal reaches saturation when probe concentration is more than 20 μmol/L.

关 键 词：寡核苷酸芯片 共价交联 玻璃 醛基修饰 载体 载玻片 制备 优化 

分 类 号：Q78[生物学—分子生物学]