豌豆根瘤菌与新疆中华根瘤菌原生质体的属间融合研究  

Study on Intergenera Fusion of Protoplasts from Rhizobium leguminosorum and Sinorhizobium xinjiangnesis

作　　者：韦革宏[1] 贺学礼 郭杰[1] 朱铭莪[1] 陈文新[2] 

机构地区：[1]西北农林科技大学资环学院,杨凌712100 [2]中国农业大学生物学院,北京100094

出　　处：《生物工程学报》2001年第5期534-538,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金 (30 0 0 0 0 5 );国家自然科学基金重点项目 (39730 0 10 )资助&&

摘　　要：以青霉素和氯霉素分别作为RhizobiumleguminosorumUSDA2 370和SinorhizobiumxinjiangnesisCCBAU110的抗药性标记。利用原生质体融合技术 ,成功地获得了USDA2 370和CCBAU110的属间融合菌株。该融合菌株可分别在双亲寄主植物上结瘤。融合菌株在细胞形态、大小、菌落特征及蛋白质电泳图谱上与亲本菌株均有所不同。融合菌株与USDA2 370的DNA同源性为 5 6 6 % ,而与CCBAU110的DNA同源性为 10 2 %。Penicillin and chloromycetin were regarded as the sign of resistance to antibiotics of R.leguminosorum USDA2370 and S.xinjiangnesis CCBAU110 respectively. Using the protoplast fusion technique, USDA2370 and CCBAU110 were successfully fused. Fusion hybrid can inoculate in the leguminous of parental strains respectively. There were apparent differences between parents and fusion hybrid in cell morphology, colony and pattern of whole\|cell protein. The values of DNA homology between fusion hybrid and USDA2370 and CCBAU110 were 56.6% and 10.2% respectively.

关 键 词：质生质体融合 豌豆根瘤菌 新疆中华根瘤菌 属间融合 

分 类 号：Q939[生物学—微生物学]