昆虫保幼激素促进家蚕杆状病毒系统的基因表达  

Insect Juvenile Hormone Enhancing Gene Expression in Silkworm Baculovirus Vector System

作　　者：王厚伟[1] 张志芳[1] 肖庆利[1] 李卫国[2] 何家禄[1] 

机构地区：[1]农业部家蚕生物技术重点开放实验室 [2]山东农业大学蚕学系,泰安271018

出　　处：《生物工程学报》2001年第5期590-593,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(39970 5 71);高技术研究发展计划项目&&

摘　　要：th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48hr after ecdysis,then treated with 100ppm Juvenile hormone.It showed that the expression level of phytase gene and polyhedrin gene per silkworm was increased by 30% and 40%,respectively.The LT 50 was lengthened for more than 4h,and the average weight of sick silkworm was increased by 10%.The results indicated that the improvement of expression efficiency of phytase gene and polyhedrin gene was mainly caused by longer time of virus replication in silkworm after the treatment of Juvenile hormone.th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48hr after ecdysis,then treated with 100ppm Juvenile hormone.It showed that the expression level of phytase gene and polyhedrin gene per silkworm was increased by 30% and 40%,respectively.The LT 50 was lengthened for more than 4h,and the average weight of sick silkworm was increased by 10%.The results indicated that the improvement of expression efficiency of phytase gene and polyhedrin gene was mainly caused by longer time of virus replication in silkworm after the treatment of Juvenile hormone.

关 键 词：家蚕 保幼激素 杆状病毒表达系统 表达效率 

分 类 号：Q78[生物学—分子生物学] S881.26[农业科学—特种经济动物饲养]