hBDNF基因在成纤维细胞中的异位表达  

Ectopic Expression of Human Brain Derived Neurotrophic Factor Gene in Fibroblasts

作　　者：刘智敏[1] 陈俊杰[2] 王若菡[2] 项涛[2] 李霞[3] 林佳[2] 游乐然[2] 东云华[2] 

机构地区：[1]华西医科大学附一院外科实验室,成都610041 [2]华西医科大学生物化学与分子生物学研究所重组DNA研究室,成都610041 [3]成都市第三人民医院药剂科,成都610013

出　　处：《生物医学工程学杂志》2001年第3期408-411,共4页Journal of Biomedical Engineering

摘　　要：将 h BDNF全长编码序列插入人 I型胶原基因 (Colia1)增强子 -启动子调节序列之下 ,构建了含 Colia1-BDNF微基因的重组体 p SCEPBFCAT,并转染人胚肌腱成纤维细胞。成功地利用了 Colia1基因调控元件介导 BD-NF基因在成纤维细胞中异位表达 ,SDS- PAGE显示表达产物分子量为 2 7k Da,免疫斑点杂交、Elisa和 Western杂交证实该表达产物具有The full length sequence encoding hBDNF was inserted down into the enhancer promotor regulatory element of Colia1 to construct minigene Colia1 BDNF. The recombinant eukaryotic expression plasmid pSCEPBFCAT was transfected into human fetal tendon fibroblasts in culture to realize ectopic expression of hBDNF gene in fibroblasts. The results of immuno dot blot, Elisa and Western hybridization confirm that the expression protein with apparent molecular weight 27 kDa in pSCEPBFCAT transfected fibroblasts possesses antigen activity specific for the antibodies to BDNF.

关 键 词：HBDNF 调控元件 微基因 成纤维细胞 基因异位表达 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]