江苏地区汉族人群六个短串联重复基因座的群体遗传学研究(英文)  

Population Genetic Studies on Six Short Tandem Repeat (STR) Loci in Han Population in Jiangsu Province, China

作　　者：易龙[1] 应锋[1] 丁卫东[1] 吴晓宇[1] 姚根宏[1] 

机构地区：[1]南京大学医学院DNA指纹实验室,南京210093

出　　处：《南京大学学报（自然科学版）》2001年第5期653-658,共6页Journal of Nanjing University（Natural Science）

摘　　要：采用复合扩增、聚丙烯酰胺凝胶电泳分型和银染显示的方法 ,对江苏地区汉族人群中 111个无关个体的 6个短串联重复 (STR)基因座 (CSF1PO ,TPOX ,TH0 1,D16S5 39,D7S82 0和D13S317)进行了群体遗传学研究。经X2 检验 ,6个基因座的基因型频率符合Hardy Weinberg平衡定律 (P >0 .0 5 )。它们的联合个体识别率 (DP)值 >0 .9999;联合非父排除率 (PE)值 >0 .9882。这 6个基因座在江苏地区汉族人群中显示了高度的多态性和良好的鉴别能力 。Allele and genotype frequencies for six terameric short tandem repeat (STR) loci were established from 111 unrelated Han individuals in Jiangsu province, China using two multiplex polymerase chain reactions (PCR), followed by eletrophoresis of the PCR products in denaturing polyacrylamide gels and subsequent detection by silver staining. The loci were CSF1PO, TPOX, TH01, D16S539, D7S820 and D13S317. All loci met Hardy-Weinberg expectations ( P>0.05 ). The discrimination power (DP) and the power of exclusion (PE) from combination of 6 STR loci were >0.999 9 and >0.988 2, respectively. Hence, the six STR loci were highly suitable for forensic individualization and paternity tests to Han population in Jiangsu.

关 键 词：短串联重复基因座 群体遗传学 聚合酶链反应 法医学 江苏 汉族 个体识别 

分 类 号：Q987[生物学—遗传学]