烟酰胺拮抗紫外线诱导的免疫抑制作用研究  

Research on Antagonism of Nictinamide in Immune Suppression Induced by UV

作　　者：王家骏[1] 谢妮[2] 刘艳[1] 孙玮[2] 李晶[2] 刘扬[2] 王秉贤[2] 于佳明[3] 

机构地区：[1]沈阳医学院环境卫生学教研室,沈阳110031 [2]中国医科大学公共卫生学院 [3]沈阳市卫生防疫站

出　　处：《中国公共卫生》2001年第6期503-505,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金!资助课题 (39570 60 6)

摘　　要：目的　探讨抗氧化剂烟酰胺拮抗紫外线诱导的免疫抑制作用。方法　用单克隆抗体免疫法、中性红比色法、MTT法检测郎格罕细胞、腹腔巨噬细胞吞噬功能、脾脏T细胞增殖功能。结果　阳性组的迟发性过敏反应受到抑制 ,其耳、背部郎格罕细胞数、脾脏T细胞增殖功能低于单纯诱导组和烟酰胺组 (P <0 0 1)。烟酰胺组出现了迟发性过敏反应 ,耳、背部郎格罕细胞数、腹腔巨噬细胞吞噬功能、脾脏T细胞增殖功能皆高于阳性对照组 ,统计学上有显著性差异 (P <0 0 1)。结论　烟酰胺具有拮抗紫外线诱导的细胞免疫抑制的作用。Objective To explore nicotinamide'antagonising immunosuppression action induced by UV.Methods We used monoclonal antibody immunifaction,neutral red colorimetric analysis,MTT method to measure the number of langerhans cells(LC),phagocytosis of macrophage in abdominal cavity and multiply function of spleen T cell.Intensity of Delayed Type Hypersensitivity(DTH) was measured with sliding caliper by us.Results In positive control group intensity of DTH was restrained.The number of LC in the ear and back of the mice and the multiply function of spleen T cell were lower than the purely induced group and nicotinamide group(P<0 01).The nicotinamide group appeared DTH and the number of LC in the ear and back,phagocytosis of macrophage in abdomical cavity,the multiply function of spleen T cell were higher than the positive control.Conclusion Nicotinamide can antagonize immunosuppression action induced by UV.

关 键 词：烟酰胺 紫外线 郎格罕细胞 腹腔巨噬细胞 脾脏T细胞 抗氧化剂 

分 类 号：R977.9[医药卫生—药品] R594.5[医药卫生—药学]