高效液相法测定八角属部分植物果实中莽草酸的含量  

Determination of Shikimic Acid in Fruit of Illiciaceae Plants by HPLC with Diode-Array Detection

作　　者：王晓强[1] 郭亚健[1] 杨春澍[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中国中药杂志》2001年第7期447-449,共3页China Journal of Chinese Materia Medica

基　　金：国家科委课题 (96 90 1 0 1 35 )

摘　　要：目的 :建立莽草酸含量测定方法。方法 :采用NH2 键合相硅胶柱 ,乙腈 2 %H3PO4 (95∶5 )为移动相 ,检测波长 2 13nm ,HPLC法。结果 :平均回收率为 98.5 % ,RSD为 1.6 7% (n =5 )。结论 :适用于含莽草酸药材及制剂的质量控制。Objective: To establish the determination method of Shikimic acid . Method: Using HPLC method as the determination method. The separation was performed in a SiO 2-NH 2 column with a mobile phase of Acetonitrile-2%H 3PO 4 water solution (95∶5);The sample wavelength was 213 nm,reference wavelength 300 nm。 Result: The average collection was 98.5%, RSD 1.67% (n=5). Conclusion: This method is suitable for the determination of Shkimic acid in herb medicines and preparation containing Shikimic acid. [

关 键 词：二极管阵列 高效液相色谱法 莽草酸 八角属 

分 类 号：R284.1[医药卫生—中药学]