镁对实验性心肌缺血再灌注时红细胞膜的保护作用  

Protective Effect of Magnesium on Red Blood Cell Membrane in Rabbit Model of Ischemia-Reperfusion

作　　者：李雪萍[1] 董果雄[1] 张社华[2] 

机构地区：[1]青岛大学医学院附属医院心内科,山东省青岛市266012 [2]青岛大学医学院中心实验室

出　　处：《中国循环杂志》2001年第3期220-222,共3页Chinese Circulation Journal

摘　　要：目的：探讨缺血再灌注时红细胞膜的功能改变及硫酸镁对其保护作用。 方法：新西兰大耳白兔３２只随机分为４组，每组８只，对照组行左冠状动脉前降支穿线，缺血组行左冠状动脉前降支持续结扎，再灌注组及治疗组结扎０．５小时后再灌注，治疗组于结扎后静脉滴注硫酸镁溶液，对照组、缺血组、再灌注组静脉滴注生理盐水；分别于手术前、缺血０．５小时、再灌注１小时及４小时取血；荧光偏振法测定红细胞膜脂流动性，比色法测定红细胞内超氧化物歧化酶、丙二醇含量，火焰原子吸收分光光度法测定红细胞内钙离子浓度。 结果：①对照组手术前后备指标差别无显著意义（Ｐ＞０．０５）。②缺血组、再灌注组及治疗组在结扎０．５小时，再灌注１小时、４小时较对照组同一时间及同组手术前相比红细胞膜荧光偏振度增加，红细胞超氧化物歧化酶减少、丙二醛增加，红细胞内钙离子增加（ Ｐ均＜ ０． ０１）。③治疗组在再灌注 １小时、 ４小时、红细胞超氧化物歧化酶量较缺血组、再灌注组明显增加，红细胞膜荧光偏振度、红细胞丙二醛、红细胞内钙离子量明显降低，差异显著（Ｐ均＜０．０１）。 结论：在缺血再灌注过程中，红细胞膜发生过氧化损伤，红细胞膜脂流动性减低。硫酸镁能减轻红细胞膜分子过氧化损伤，改善红细?Objective: To investigate red blood cell membrane function during coronary ischemia-reperfusion injury and the protective effect of supplement of magnesium. Methods: Thirty-two rabbits were randomly divided into four groups. In group I the left anterior descending coronary artery (LAD)was falsely ligated. In group Ⅱ the LAD was ligated. Rabbits of group Ⅲ and Ⅳ underwent 0.5h of LAD oc- clusion and 4h of reperfusion. LAD-ligated rabbits in group Ⅳ were intravenously given magnesium and in group Ⅰ, Ⅱ, Ⅲ sodium chloride. Red blood call membrane fluidity, erythrocyte content of superoxide dismutase, malondialdehyde, calicum were determined at some points before and after operation. Results: ①There was no significantly different in group Ⅰ before and after operation (p>0.05). ②In group Ⅱand Ⅲ, red blood cell membrane fluidity, erythrocyte superoxide dismutase (SOD)content were decreased significantly and the erythro- cyte MDA, Ca2+ content were increased after myocardial ischemia/ischemia-reperfusion (p < 0. 0 1 ). ③ Erythrocyte membrane fluidity, erythrocyte SOD content were accelerated and the erythrocyte MDA, Ca2+ content were decreased when the magnesium sulphate was administered after the ligation (p<0.01). Conclusion: Our studies suggest that magnesium has beneficial effects on red blood cell in myocardial ischemia-reperfusion.

关 键 词：心肌缺血再灌注 镁钙 红细胞膜 实验研究 

分 类 号：R542.2[医药卫生—心血管疾病]