小剂量雌激素对去卵巢骨质疏松大鼠骨小梁结构的影响  

Effects of Lower Dose of Estrogen on Trabecular Structure of Femur in Ovariectomized- induced Osteoporosis Rats

作　　者：孙兰[1] 杨京[1] 刘景生[1] 

机构地区：[1]中国医学科学院 中国协和医科大学 基础医学研究所药理室,北京100005

出　　处：《中国医学科学院学报》2001年第3期224-227,I001,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金( 39970856)资助&&

摘　　要：目的 观察小剂量雌激素对去卵巢骨质疏松大鼠血清雌二醇（ E2）浓度和骨小梁结构的影响。方法 12月龄雌性大鼠去除双侧卵巢建立骨质疏松模型。实验分四组：假手术组、去卵巢骨质疏松组（ OVX）、 E2替代治疗组 [2.0 mg /(kg· d)]和小剂量 E2组 [0.5 mg/(kg· d)]。去卵巢术 2周后开始灌胃给 E2，连续 12周。在实验结束前 2周，对所有大鼠进行阴道脱落细胞检查，连续 14 d。用显微镜观察涂片，并根据细胞形态和数量判断大鼠动情周期。给药结束后处死动物，取子宫，称其湿重。取全血，采用放射免疫分析法测定血清 E2、卵泡刺激素（ FSH）及黄体生成素（ LH）浓度。通过股骨骨病理切片观察骨小梁结构。 结果 与 OVX组大鼠相比，小剂量 E2组大鼠股骨远端小梁骨粗壮，骨质密度较高，作用与 E2替代组接近，但大鼠子宫重量、血清 E2浓度和动情周期无明显改变。 结论 小剂量 E2能明显改善去卵巢骨质疏松大鼠的骨小梁结构，同时对生殖系统无影响。Objective To study the effects of lower dose of estrogen on the serum estrodial concentration and trabecula structure of femur in ovariectomized (OVX)- induced osteoporosis rats. Methods OVX- induced osteoporosis model was established by both sides of ovariectomy in 12- month- old female rats. All rats were divided into four groups: sham- operated group, OVX- induced osteoporosis group, E2- replacement- therapy (ERT) group [E2 2.0 mg/(kg· d)], and lower dose E2- treated (LDET) group [E2 0.5 mg/(kg· d)]. Two weeks after the operation, E2 was given to the rats for 12 weeks through stomach. The estrus was detected by means of vagina smear. Then, all rats were killed and the uterines were weighted. Serum E2, FSH, and LH concentrations were measured by radioimmunoassay. Bone structure was observed by light microscopy of right femur sections. Results In the LDET rats, the trabeculae were thick and large, the bone densities were higher than that of OVX rats, which approached to that of ERT rats. However, there were no changes in the uterine weight, serum E2 concentration， and estrus in those rats. Conclusion LDET improved trabecular structure in OVX- induced osteoporosis rats while the reproductive system was not affected.

关 键 词：雌激素 去卵巢 骨质疏松 骨小梁 实验研究 

分 类 号：R977.1[医药卫生—药品] R681[医药卫生—药学]