尼可地尔超极化心脏停搏心肌保护的实验研究  

Experimental study on myocardial protection:hyperpolarized cardioplegic arrest with nicorandil

作　　者：周志有[1] 景华[1] 张石江[1] 李德闽[1] 李忠东[1] 周建峰[1] 顾卫东[1] 高声甫[1] 

机构地区：[1]南京军区南京总医院心胸外科,210002

出　　处：《江苏医药》2001年第9期665-667,共3页Jiangsu Medical Journal

基　　金：国家人事部科研基金类资助项目 ( 19982 87)

摘　　要：目的　比较高钾心停搏液去极化心脏停搏和尼可地尔心停搏液超极化心脏停搏的心肌保护效果。方法　实验分去极化心脏停搏组和超极化心脏停搏组 (每组动物 8只 )。 4℃心停搏液(40ml/kg)诱停离体鼠心 ,每 30分钟重复灌注半量心停搏液 (2 0ml/kg) ,15± 1℃心脏停搏 12 0分钟。记录心脏机械停搏时间、再灌注后心脏复跳情况、心功能指标恢复、冠状循环灌注平均流量、心肌丙二醛 (MDA)含量及心肌细胞超微结构的改变。结果　超极化心脏停搏组对心功能恢复、冠状循环灌注平均流量及心肌组织超微结构的保护效果优于去极化心脏停搏组 (P <0 0 5 ) ,心肌MDA含量低于去极化心脏停搏组 (P <0 0 1)。结论　尼可地尔超极化心停搏液 (10 0mmol/L)Objective To evaluate myocardial protective effect of hyperpolarized cardioplegic arrest,depolarized cardiac arrest with St.Thomas′ No.2 solution and hyperpolarized cardiac arrest with Nicorandil were compared in isolated working rat heart.Method Sixteen isolated working rat hearts were divided into 2 groups randomly.They were group A:depolarized cardiac arrest with St.Thomas′ solution No.2,group B:hyperpolarized cardiac arrest(Nicorandil 100μmol/L).The hearts underwent a 120 minute hypothermic arrest(15±1℃) with induction of cardioplegia(40ml/kg) and reinfusion of cardioplegia(20ml/kg) at interval of 30 minutes.Mechanical arrest time,cardiac functional recoveries,myocardial content of malondialdehyde(MDA) and ultrastructure were measured.Results There were statistically significant differences between A and B groups in the recovery of cardiac function,myocardial content of MDA,and ultrastucture of mitochondrial membranes and cristae.Conclusion The effect of myocardial protection with nicorandil is superior to that with St.Thomas′ No.2 solution.

关 键 词：尼可地尔 心肌保护 超极化心脏停搏 ATP敏感钾通道开放剂 

分 类 号：R972[医药卫生—药品] R654.1[医药卫生—药学]