应用组织培养的甲型肝炎病毒抗原作为酶联免疫诊断试剂  

AN HEPATITIS A ELISA KIT WITH TISSUE CULTURE HEPATITIS A VIRUS ANTIGEN

作　　者：尹卫东[1] 龚伯玲[1] 

机构地区：[1]唐山市卫生防疫站

出　　处：《病毒学报》1989年第1期58-61,共4页Chinese Journal of Virology

摘　　要：用2BS细胞培养甲型肝炎病毒(HAV)TZ84株,一次性加维持液,仅培养8～10天即能稳定地大量地生产甲型肝炎病毒抗原(HAAg),可用于制备甲型肝炎酶联免疫诊断试剂。采用竞争法检测HAV抗体仅需10μl血清,1～2小时即可得出结果,与Abbott试剂相比,符合率为95％,无假阳性出现。方法简便、快速,适用于人群甲型肝炎病毒感染状况的调查,采用抗体捕捉法测抗-HAV IgM,与Abbott试剂相比,符合率为96.98％,无假阳性出现,适用于甲型肝炎的早期诊断,用于临床和流行病学调查,表明所研制的甲型肝炎诊断试剂可靠实用。TZ84 strain of HAV was inoculated onto human diploid cell strain 2BS and cultured for 8-10 days. Hepatitis A Antigen ( HAAg ) was extracted from the infected cells. It is used for preparation of HAV diagnosis ELISA kit. The competitive ELISA method was for the detection of Anti-HAV. It is simple, quick and needs only very little sample ( 10 μl only) . The result can be read within 1-2 hours. The coincident rate with Abbott kit was 95%. There were no false positive. The capture ELISA method was used for the detection of Anti-HAV IgM. The coincident rate with Abbott kit was 97%, also with no false positive. This article also describes the results of application of these reagents to the clinical diagnosis and epidemi-ological survy of hepatitis A.

关 键 词：甲型肝炎抗原 ELISA 组织培养 

分 类 号：R374.21[医药卫生—病原生物学]