虎杖甙对脂多糖诱导血管内皮细胞ICAM-1表达的抑制作用  

Inhibiting role of polydatin to expression of intercellular adhesion molecule-1 induced by lipopolysaccharide on vascular endothelial cells

作　　者：闫文生[1] 赵克森[1] 姜勇[1] 王静珍[1] 

机构地区：[1]第一军医大学病理生理教研室,广东广州510515

出　　处：《中国病理生理杂志》2001年第9期855-857,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金重点资助项目 (No.39830 40 0 )

摘　　要：目的和方法 :用细胞免疫荧光染色和激光共聚焦显微镜扫描技术 ,研究脂多糖 (LPS)对人脐静脉内皮细胞(HUVEC)细胞间粘附分子 - 1(ICAM - 1)表达的诱导作用 ,以及虎杖甙对ICAM - 1表达的抑制作用。结果 :内皮细胞未受LPS剌激时 ,细胞表面ICAM - 1表达很弱 ;LPS剌激后 ,细胞表面ICAM - 1分子在第 8- 36h显著增加 ,并与ICAM - 1的表达呈剂量反应关系。虎杖甙单独处理内皮细胞时 ,对ICAM - 1表达无明显影响 ;但虎杖甙预处理内皮细胞后 ,可显著抑制LPS对ICAM - 1表达的诱导作用。结论 :LPS可促进内皮细胞ICAM - 1的表达 。AIM and METHODS: To investigate expression of intercellular adhesion molecule-1(ICAM-1) on human umbilical vein endothelial cells (HUVEC) induced by lipopolysaccharide (LPS) , and inhibiting role of polydatin by cellular immune fluorescent staining and laser confocal microscope scanning technology. RESULTS: Compared with basic expression of ICAM-1 on HUVEC, the ICAM-1 expression was enhanced significantly after stimulated by LPS from 8 h to 36 h, dose-dependent relation appeared between expression of ICAM-1 and LPS. ICAM-1 expression on endothelial cells treated only by polydatin had no abvious change,but inducing role of LPS to expression of ICAM-1 was inhibited significantly by polydatin pretreating endothelial cells. CONCLUSION: The expression of ICAM-1 on endothelial cells can be promoted by LPS , and polydatin can inhibit LPS-induced ICAM-1 expression

关 键 词：脂多糖类 血管 细胞粘着分子 内皮细胞 ICAM-1 中药 虎杖甙 抗休克治疗 抑制作用 

分 类 号：R282.71[医药卫生—中药学]