路盖克片中对乙酰氨基酚和酒石酸双氢可待因含量测定HPLC法的改进  

Improved HPLC determination of paracetamol and dihydrocodeine tartrate in Galake tablets

作　　者：刘建春 栾成章 王立 庞靖 

机构地区：[1]青岛市药品检验所,青岛266071

出　　处：《中国新药杂志》2001年第8期59-60,共2页Chinese Journal of New Drugs

摘　　要：目的 :采用RP HPLC法 ,同时测定路盖克片中对乙酰氨基酚和酒石酸双氢可待因的含量。方法 :使用C18柱 (2 0 0mm× 4.6mm ,5 μm) ,柱温 5 0℃ ,流动相为乙腈 pH3.0缓冲液 (1%NH4 H2 PO4 +0 .0 5mol·L-1庚烷磺酸钠 ) (15∶85 ) ,检测波长分别为 2 80和 2 0 9nm ,灵敏度分别为 0 .1和 0 .0 1AUFS ,流速 2 .0mL·min-1,进样量 2 0 μL。结果 :能同时测定对乙酰氨基酚和酒石酸双氢可待因的含量 ,线性范围分别为 5 5～ 2 76 μg·mL-1(r =0 .9999)和1.1～ 5 .9μg·mL-1(r=0 .9999) ;平均回收率分别为 10 0 .2 % (RSD =0 .9% )和 99.7% (RSD =1.9% )。结论 :操作简单、快速 。Objective:To improve the HPLC method for simutaneous determination of paracetamol and dihydrocodeine tartrate in Galake tablet.Methods:A C 18 column(200mm×4.6mm,5μm),a column temperature of 50℃,a mobil phase composed of acetonitrile and a pH 3.0 buffer solution (1% NH 4H 2PO 4+0.05mol·L -1 sodium 1 heptanesulfonate)(15∶85) were adopted.The instrument parameters were set below wave length:280 nm,209 nm;sensitivity:0.1,0.01 AUFS and flow rate 2.0 mL·min -1 .Results:The linear range for paracetamol and dihydrocodeine tartrate were 55～276 μg·mL -1 ( r =0.9999)and 1.1～5.9μg·mL -1 ( r =0.9999)respectively.The average recovery for paracetamol and dihydrocodeine tartrate were 100.2% ( RSD =0.9%)and 99.7%( RSD =1.9%) respectively.Conclusion:The method is simple and rapid with results similar to that of the oringinal method.

关 键 词：对乙酰氨基酚 酒石酸双氢可待因 反相高效液相色谱法 路盖克 

分 类 号：R971.1[医药卫生—药品]