脊髓半横断损伤后大鼠皮层神经元细胞骨架蛋白基因表达的变化  

CHANGES OF CYTOSKELETAL GENES EXPRESSION IN CORTICOSPINAL NEURONS AFTER SPINAL CORD SEMITRANSECTION INJURY IN RAT

作　　者：米瑞发[1] 周长满[1] 范明[2] 

机构地区：[1]北京军医学院,北京100071 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《解剖学报》2001年第4期329-333,T008,共6页Acta Anatomica Sinica

基　　金：国家 973资助项目 (G19990 5 40 0 0 )

摘　　要：目的　探讨神经再生过程中细胞骨架蛋白的作用机制 ,了解中枢和外周神经系统神经元应答神经损伤和再生的差异。　方法　用原位分子杂交方法观察了大鼠T10～ 11脊髓半横断损伤后 1、3、5、7、10、14、2 1、2 8、5 6d皮层感觉运动区皮层神经元中α 管蛋白和 3个神经丝蛋白亚基mRNA的表达变化。　结果　脊髓半横断损伤后损伤侧皮层感觉运动区皮层神经元中α 管蛋白、神经丝 L、 M、 HmRNA表达明显下调。α 管蛋白mRNA表达水平在损伤后 1d降低 ,而神经丝亚基mRNA表达水平直到下一个时间点 (损伤后 3d)才下调 ,至损伤后 5 6d均无恢复趋势。结论　皮层神经元损伤后α 管蛋白mRNA的表达被抑制 。Objective To investigate the pattern and the role of cytoskeletal genes expression during nerve regeneration. Methods We examined the changes of expression of α tubulin(α Tub)and three neurofilament(NF)subunits mRNA in rat corticospinal neurons of sensorimotor cortex on the 1st,3rd,5th,7th,10th,14th,21st,28th and 56th days after spinal cord semitransection injury by using in situ hybridization. Results Levels of α Tub,NF L,NF M and NF H mRNA were dramatically downregulated within large sized pyramidal neurons in layer V of the sensorimotor cortex of side lateral after spinal cord injury.The level of α Tub mRNA was decreased as early as 1 day postinjury,whereas the levels of NF subunits mRNA were not decreased until later time(on the 3rd days postinjury),and both of them did not return to control levels on the 56th days following the lesion. Conclusion α Tub gene expression if inhibited in CNS neurons postinjury. The axotomy signeal and the continuance downregulation of NF mRNA expressioon following spinal cord injury may contribute to the ineffective regeneration response of CNS neurons.

关 键 词：管蛋白 神经丝 基因表达 皮层神经元 脊髓损伤 大鼠 细胞骨架蛋白 

分 类 号：R651.2[医药卫生—外科学]