Smad2和Smad4蛋白在成年大鼠睾丸的表达  

EXPRESSION OF SMAD 2 AND SMAD 4 PROTEINS IN THE ADULT RAT TESTIS

作　　者：胡静[1] 张远强[1] 王红[1] 张金山[1] 许若军[2] 

机构地区：[1]第四军医大学组织学胚胎学教研室,西安710032 [2]香港大学动物学系

出　　处：《解剖学报》2001年第4期354-356,T015,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9870 10 9);全军医药卫生科研基金资助项目 (98M10 6)

摘　　要：目的　探讨转化生长因子 β超家族肽类的细胞内信号转导分子Smad 2和Smad 4蛋白在成年大鼠睾丸的表达及在精子发生中的作用机理。　方法　用免疫组织化学ABC法结合葡萄糖氧化酶 DAB 硫酸镍铵增强技术 ,检测成年大鼠睾丸Smad 2和Smad 4蛋白的表达和定位。　结果　Smad 2蛋白主要表达在大鼠睾丸生精小管的精原细胞、初级精母细胞、次级精母细胞、精子细胞、支持细胞和间质细胞中 ,免疫反应物质主要定位于细胞质 ,胞核为阴性 ;而Smad 4蛋白则主要表达于间质细胞的胞质中 ,支持细胞呈弱阳性染色。　结论　Smad 2蛋白主要表达于睾丸生精小管各级生精细胞 ,Smad 4蛋白表达于间质细胞 ,为揭示TGFObjective To study the localization of Smad 2 and Smad 4 proteins, which are intracellular signaling molecules of transforming growth factor β family in adult rat testis. Method Immunohistochemical ABC method with glucose oxidase DAB nickel enhancement technique was used in the present study. Results Smad 2 immunoreactivity was mainly located in the spermatogonia, spermatocytes, spermatids, Sertoli cells in the seminiferous tubule and Leydig cells in the interstitial tissue. The reactive substance distributes in cytoplasm with negative nuclei. While Smad 4 is mainly expressed in cytoplasm of Leydig cells, and Sertoli cells is weak stained. Conclusion Our findings of Smad 2 expression in spermatogenic cells and Smad 4 expression in Leydig cells provide direct evidence for the molecular mechanism of TGF β action during spermatogenesis.

关 键 词：TGF-Β SMAD2 SMAD4 精子发生 免疫组织化学 大鼠 睾丸 

分 类 号：Q959.837[生物学—动物学] Q954.434