谷氨酸与代谢型谷氨酸受体1亚型、2/3亚型、4亚型在参环毛蚓的分布——免疫细胞化学研究  

IMMUNOCYTOCHEMICAL LOCALIZATION OF GLUTAMATE AND METABOTROPIC GLUTAMATE RECEPTOR,mGluR1, mGluR2/3, mGluR4 IN EARTHWORM, P. ASPERGILLUM

作　　者：罗振国[1] 张小云[1] 

机构地区：[1]深圳大学生命科学研究所,深圳518060

出　　处：《解剖学报》2001年第4期375-377,T018-T019,共5页Acta Anatomica Sinica

摘　　要：目的　探讨谷氨酸与其代谢型谷氨酸受体在参环毛蚓 (P .aspergillum)的存在与否及分布情况。方法　用免疫细胞化学染色技术 ,在光学显微镜下观察谷氨酸与代谢型谷氨酸受体 1亚型、2 / 3亚型、4亚型阳性细胞的形态与分布。　结果　发现谷氨酸存在于参环毛蚓的脑、咽下神经节、腹部神经节的神经细胞、肌间神经细胞 ,以及肠上皮和体表上皮的非神经细胞。在围咽神经环与腹神经链及腹神经链所发出侧支的切面可见深染的细密的阳性神经纤维束。代谢型谷氨酸受体 1亚型、4亚型仅存在于参环毛蚓的脑。未观察到代谢型谷氨酸受体 2 / 3亚型阳性细胞的存在。　结论　参环毛蚓中枢神经系统谷氨酸阳性神经细胞的部分细胞突起显著 ,形态成熟 ,围咽神经环与腹神经链及腹神经链所发出侧支的阳性神经纤维是它们的投射纤维 ;部分细胞无突起 ,形态幼稚 。Objective Our purpose is to explore the occurrence and distribution of glutamate and metabotropic glutamate receptor in earthworm, P. aspergillum and to analyze their function significance. Methods By employing immunocytochemistry tchnique, the positive cells of glutamate and metabotropic glutamate receptor, mGluR1, mGluR2/3, mGluR4 in earthworm, P. aspergillum were investigated under a light microscope. Results It was found that glutamate positive cells existed in cerebroganglion, subpharyngeal ganglion, ventral ganglion, neurons between muscle cells,intestinal epithelium and epidermis, that thin and dense glutamate positive fibers distributed in pharyngeal nerve cycle, subpharyngeal ganglion, ventral neurochain, that mGluR1 or mGluR4 positive neurons were detected in cerebroganglion and mGluR2/3 positive neurons weren't observed in earthworm. Conclusion During glutamate positive neurons, some possess remarkable processes and matured appearance from which glutamate positive fibers in pharyngeal nerve cycle, subpharyngeal ganglion, ventral neurochain derive, another have few branches, unmatured appearance in which glutamate doesn't serve as neurotransmitter acting to mGluR1 or mGluR4 of neurons in earthworm cerebroganglion.

关 键 词：谷氨酸 代谢型谷氨酸受体 免疫细胞化学 参环毛蚓 亚型 

分 类 号：Q954.62[生物学—动物学] R392[医药卫生—免疫学]