白细胞介素2提高HBV-SDNA疫苗的免疫效应  

Interleukin 2 eukaryotic expression plasmid promoted the specific immune responses induced by hepatitis B DNA vaccine

作　　者：杜德伟[1] 周永兴[1] 白宪光[1] 刘清泉[1] 陈红梅[1] 李谨革[1] 连建奇[1] 冯志华 姚志强[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,陕西西安710038

出　　处：《西北国防医学杂志》2001年第3期234-236,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：国家自然科学基金资助项目 ( 39770 665 )

摘　　要：目的 :观察IL -2真核表达载体对HBV SDNA疫苗诱导BALB/c小鼠的特异性免疫应答的影响。方法 :肌肉注射基因疫苗及IL -2真核表达载体 ,ELISA法检测小鼠血清抗HBs,4h5 1Cr释放法检测小鼠脾细胞CTL活性。结果 :免疫 8周后 ,单纯注射pCR3 .1 -S及共注射IL -2真核表达载体的小鼠血清 4 50nmA值分别为 1 .2 4± 0 .1 0及 1 .98± 0 .1 7。CTL细胞杀伤活性分别为 ( 50 .5± 6.4 ) %及 ( 61 .9± 7.1 ) % ,两组均有明显差异 (P <0 .0 1 )。结论 :IL -2的真核表达载体能够提高小鼠对DNA疫苗的免疫应答。基因疫苗可能用于预防及治疗HBV感染。Objective:To observe the effect of interleukin 2 eukaryotic expression plasmid on specific immune responses to gene vaccines coding HBV surface antigen in Balb/c(H-2 d) mice.Methods:The immunization was performed by intramuscular injection in this experiment.Anti-HBs in serum was detected by ELISA.HBsAg specific cytotoxic T lymphocytes(CTLs) activity was measured by 51 Chromiun release assay.Results:8wks after immunization,the serum of mice A value in 450 nm codeliveried IL-2 vaccine(1.98±0.17) was significant higher than that of mice injected HBV-S DNA vaccine(1.24±0.10).CTLs activity of the mice injected HBV-S DNA vaccine and codeliveried IL-2 vaccine were (50.5±6.4)% and (61.9±7.1)% respectively.Conclusion:The results showed that the DNA vaccine of pCR3.1-S had strong antigenecity in cellular and humoral immunity which can be promoted by vector coding murine IL-2.DNA vaccine against HBV may be useful for both prophylactic and therapeutic purposes.

关 键 词：DNA疫苗 乙型肝炎表面抗原 IL-2 免疫应答 乙型肝炎 

分 类 号：R512.6[医药卫生—内科学]