程控降温后液氮保存对自体外周血造血干细胞含量和活性的影响  

The effects of programmed freezing and liquid nitrogen storage on stem cells quantity and vitality

作　　者：路继红[1] 欧英贤[1] 白海[1] 魏亚明[1] 王存邦[1] 吕桂玲[1] 

机构地区：[1]兰州军区兰州总医院血液科,甘肃兰州730050

出　　处：《西北国防医学杂志》2001年第3期245-247,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的 :观察程控降温后液氮保存对自体外周血干细胞含量和活性的影响。方法 :1 3例接受APBSCT患者分离的造血干细胞利用程控降温仪进行冷冻 ,然后置液氮液相内 ( -1 96℃ )保存 ,检测患者冻存前后的有核细胞数 ,CFU -GM ,CD3 4+细胞。结果 :冻存前后CFU -GM ,有核细胞数 ,CD3 4+细胞统计学之间无显著性差异。结论 :自体外周血干细胞体外冷冻处理和保存是有效和适当的。Objective:To investigate effects of programmed freezing and liguid nitrogen storage on number of auto-peripheral stem cells and their vitality.Methods:Auto-peripheral stem cells from 13 APBSCT recipients were programmatical freezed and stored in liquid nitrogen, the mononudeus cells (MNC) CFU GM CD34+cells were examined as well.Results:MNC,CFU GM,CD34+cells showed no difference between pre and post freezing.Conclusion:Pregrammed freezing and liguid nitrogen storage of stem cells are effective and suitable for APBSCT.

关 键 词：造血干细胞 自体外周血干细胞 液氮保存 程控降温 APBSCT 

分 类 号：R457.7[医药卫生—治疗学]