2-氯-4-硝基苯-β-D-半乳糖麦芽二糖苷作底物测定淀粉酶总活性  

Total amylase measured with 2-chloro-4-nitrophenyl-4-O-β-D-galaotopyranosylmalto side

作　　者：张厚亮[1] 张伟[2] 帅真[3] 张济愈 徐美宝 

机构地区：[1]天津市静海县医院检验科,天津301600 [2]天津医科大学附属肿瘤医院检验科 [3]天津市临床检验中心 [4]天津程林医院

出　　处：《临床检验杂志》2001年第5期266-268,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　建立 2 氯 4 硝基苯 β D 半乳糖 麦芽二糖苷 (GalG2 CNP)作底物测定淀粉酶的方法。方法　用连续监测法对pH值 ,Km值及最适底物浓度等反应条件进行实验研究 ,并对建立的方法进行评价。结果　用双试剂连续监测法 ;试剂Ⅰ :含KSCN 2 0 0mmol/L ,CaCl2 6 .7mmol/L ,NaCl 40 0mmol/L ;试剂Ⅱ :含GalG2 CNP 2 0mmol/L ;均用pH6 .0 5 0mmol/LMES缓冲液。线性范围达 12 0 0U/L批内CV =2 .77% ,批间CV =3 .5 6 % ,与参考方法 (EPS -G7)有良好的相关性。结论　本法操作简便 ,结果准确 。Objective To establish a new continuously monitoring assay for amylase activity in serum and ruine. Methods Using 2 chloro 4 nitrophenyl 4 O β D galctopyranosylmaltoside (GalG2CNP)as the substrate; amylase converted GalG 2CNP into β D galaotopyranosylmaltose. And 2 chloro 4 nitrophenol, which was measured at 405 nm. Results The linearity range of this method can reach 1200 U/L. The variance within batch and between batch were 2.77% and 3.56% respectively. Test results compare closely with the IFCC method (EPS-G7). Conclusion The method is easily automated and is suitable for routine work in clinical laboratories.

关 键 词：2-氯-4-硝基苯-β-D-半乳糖-麦芽二糖苷 连续监测法 淀粉酶 血清 尿液 

分 类 号：R446.1[医药卫生—诊断学]