应用电镜技术对一种丹顶鹤病毒的形态学观察  

MORPHOLOGICAL OBSERVATIONS BY ELECTRON MICROSCOPY OF A VIRAL AGENT ISOLATED FROM JAPANESES CRANES ( GRUS JAPONENSIS )

作　　者：俞荣林[1] 高锦梁[1] 雷兆寿 王永坤[1] 

机构地区：[1]江苏农学院

出　　处：《病毒学报》1989年第1期83-85,共3页Chinese Journal of Virology

摘　　要：1982年,作者以3只急性死亡丹顶鹤的肝脏病料感染雏鹅和成年鹅,引起雏鹅和部份成年鹅发病,剖检发现死鹅和死鹤的病变相似。而且死鹤的肝脏病料还能使鹅胚死亡,使鹅胚原代成纤维细胞和鸭胚原代成纤维细胞产生明显的病变。本实验的目的是利用超簿切片技术对病毒讲行初步鉴定。并观察其形态及发生。Electron microscopic observations of duck embryo fibroblast ( DEF ) infected with the liver material from dead Japanese cranes ( Grus japonen-sis ) revealed a virus with many empty capsids and nucleocapsids forming crystal line structures in the nucleus. The nucleocapsids were releasing from the nucleus by budding to form intracellular or extracellular enveloped vi-rions. The empty capsids and nucleocapsids were in the shape of hexagon, 95-105nm in diameter, while the enveloped virions, sphere or ellipse, were 120-180nm in diameter. The virus could induce cytopathic effects in DEF.It is inferred from the above that the virus is a member of herpesvirus.

关 键 词：疱疹病毒 丹顶鹤 形态学 电镜 

分 类 号：R852.65[医药卫生—航空、航天与航海医学]