用单克隆抗体分析流行性出血热病毒的核蛋白抗原位点  

ANTIGENiC EPITOPE ANALYSIS OF THE NUCLEOCAPSID PROTEIN OF EPIDEMIC HEMORRHAGIC FEVER VIRUS USING MONOCLONAL ANTIBODIES

作　　者：梁米芳[1] 宋干[1] 杭长寿[1] 李德新[1] 李盈盈[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1989年第1期24-30,共7页Chinese Journal of Virology

摘　　要：17株针对流行性出血热病毒(EHFV)L99株和C4株核蛋白(50kd)的单克隆抗体(McAb),根据抗原性分析可分为组特异性、型特异性(野鼠型或家鼠型),以及反应谱不全的亚组特异性McAb。这表明,在EHFV核蛋白上不仅有组特异性抗原决定簇,而且有型特异性抗原决定簇,采用竞争性酶联免疫吸附试验,以亲和层析纯化的核蛋白傲为抗原,用这些McAb分析L99株和C4株核蛋白的抗原位点,在两株病毒核蛋白上各发现了至少7个共有的位点,3个野鼠型和两个家鼠型病毒特有的位点,组特异性抗原位点至少分布在两个独立的区域,其构象和分布在不同毒株表现不尽相同,无论野鼠型或家鼠型病毒,其型特异性抗原位点均较集中在一个独立区域内。Seventeen monoclonal antibodies ( McAbs ) specific for the nucleocapsid protein ( NP ) of epidemic hemorrhagic fever virus ( EHFV ) strains L99 and C4 have been obtained. Group-specific and type-specific McAbs were identified by antigenic reactivity analysis. It was demonstrated that there were not only group-specific but also type-specific antigenic determinants on the NP of EHFV. An analysis of the antigenic epitopes of strains L99 and C4 NP was carried out by competitive ELISA using these McAbs and NP purified by affinity chromatography as antigens. At least seven. epitopes common to both the Rattus-type and Apodemus-type exist. In addition, two epitopes specific for the Rattus-type virus and three specific for the Apodemus-type virus were defined on the NP of both strains. Group specific antigenic epitopes common to both types were distributed in two major regions, its conformation and topological distribution being some what different for different EHFV strains. The type-specific antigenic epitopes of either the Rattus-type virus or the Apodemus-type virus were localized in a distinct region.

关 键 词：出血热病毒 单克隆抗体 位点分析 

分 类 号：R374.32[医药卫生—病原生物学]