联用抗口蹄疫病毒重组多肽及DNA疫苗诱发豚鼠产生特异免疫应答  

The Use of Combination Recombinant Polypeptide and DNA Vaccines Against Foot-and-mouth Disease Virus Induces Specific Immune Responses in Guinea Pigs

作　　者：李光金[1] 吴永庆[1] 严维耀[1] 徐泉兴 盛祖恬[3] 尤永进 葛燕 郑兆鑫[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433 [2]上海农业科学研究院兽医研究所,上海201106 [3]浙江省农业科学院病毒研究所,杭州310021

出　　处：《高技术通讯》2001年第6期1-4,共4页Chinese High Technology Letters

基　　金：863计划资助项目 ( 863 19972 10 )

摘　　要：选择一株O型口蹄疫病毒 (FMDV)外壳蛋白VP1中 2 1～ 40位及 1 41～ 1 60位氨基酸残基两个抗原表位 ,组成串联结构 1 41～ 1 60 ( 2 0AA) 2 1～ 40 ( 2 0AA) 1 41～ 1 60( 2 0AA) ,并与大肠杆菌β 半乳糖苷酶相连 ,在大肠杆菌中表达了此融合蛋白。同时将编码此融合蛋白的基因克隆进真核表达载体中 ,构建成重组表达质粒 pCDM8FZ1。将pCDM8FZ1DNA与融合蛋白混合 ,免疫豚鼠一次 ,观察豚鼠产生免疫应答情况。结果显示 ,豚鼠可产生抗FMDV中和抗体及特异性T细胞增殖反应 ,部分豚鼠能抵抗FMDV的攻击 ,保护率为 50 % ,证明将DNA疫苗与重组多肽疫苗结合起来作一次免疫 ,是可望用于预防FMDV感染的新途径 ,值得进一步探索。Two epitopes within VP1 of a type O foot and mouth disease virus(FMDV), residues of amino acids 21～40 and 141～160, were ligated into a tandem repeat 141～160(20AA) 21～40(20AA) 141～160(20AA). This tandem sequence was fused with β galactosidase (β gal) to form a fusion protein. In addition, the DNA fragment coding for this fusion protein was cloned into an eukarytic expression vector to generate a recombinant expression plasmid designated pCDM8FZ1. The mixture of pCDM8FZ1 DNA and the fusion protein was inoculated intramuscularly a single time into guinea pigs. The immune responses induced by the mixed vaccines were assayed. The results showed that the FMDV neutralizing antibody and specific T cell proliferative response were generated in immunized animals, and 50% of guinea pigs were protected from viral challenge. The study demonstrated that it is a promising strategy to prevent FMDV infection by vaccination a single time with DNA plus recombinant polypeptide vaccines, which warrants further active investigation.

关 键 词：口蹄疫病毒 DNA疫苗 苗组多肽疫苗 免疫应答 预防 口蹄疫 大肠杆菌 免疫接种 

分 类 号：S855[农业科学—临床兽医学]