混浊介质多光子激发荧光显微成像的蒙特卡罗模拟  

Monte Carlo Simulation for MPE Fluorescence Microscopy in Turbid Media

作　　者：鲁强[1] 曾绍群[1] 骆清铭[1] 阮玉[1] 

机构地区：[1]华中理工大学生物医学光子学教育部重点实验室,武汉430074

出　　处：《光学学报》2001年第9期1073-1078,共6页Acta Optica Sinica

基　　金：国家自然科学基金 (39870 2 0 5;5 9836 2 40;6 0 0 2 5 5 14);高校博士点专项基金 (980 48733)资助课题

摘　　要：将蒙特卡罗方法与几何光线追踪技术相结合用于研究样品混浊特性对多光子激发荧光显微成像的影响。推导了模拟多层样品显微成像的数学模型 ,给出了用程序框图表示的基本仿真系统骨架。对传统蒙特卡罗方法的改进有效地增强了对聚焦光束的模拟能力。A numerical program was presented, which combines Monte Carlo method with geometrical ray trace used to study the behaviour of multiphoton excitation (MPE) fluorescence microscopic imaging through turbid media. The mathematical model used to microscopically image multilayered tissues was established. The basic skeleton of the simulation program was demonstrated with flow chart. A skill was introduced to improve the traditional Monte Carlo method, which enhances the capability of simulation of convergent light. The prominent effect on microscopic imaging caused by scattering characteristics of turbid media was shown by preliminary simulation experiments.

关 键 词：多光子激发 荧光显微术 蒙特卡罗方法 混浊介质 成像技术 

分 类 号：TN911.73[电子电信—通信与信息系统] O242.2[电子电信—信息与通信工程]