原因不明习惯性流产患者主动免疫治疗前后T辅助细胞1、2型细胞因子的变化  

Study on changes of serum T helper cell type 1 and 2 cytokines after active immunotherapy in women with unexplained habitual abortion

作　　者：邱丽华[1] 林其德[1] 洪燕[1] 李东至[1] 丁传伟[1] 

机构地区：[1]上海第二医科大学附属仁济医院妇产科,200001

出　　处：《中华妇产科杂志》2001年第7期408-410,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：卫生部科学研究基金资助 ( 98 2 30 1)

摘　　要：目的　探讨原因不明习惯性流产 (UHA)患者主动免疫治疗前后血清T辅助细胞 (TH) 1/TH2型细胞因子的变化。方法　采用酶联免疫吸附法检测 3 0例正常非妊娠妇女 (正常非孕组 )、3 0例正常妊娠妇女 (正常妊娠组 )和 3 3例UHA患者 (流产组 )淋巴细胞主动免疫治疗前后血清TH1型细胞因子 [白细胞介素 (IL) 2、IL 12、γ干扰素 ]和TH2型细胞因子 (IL 4、IL 10、转化生长因子β1)水平。 结果( 1)流产组治疗前血清IL 2、IL 12的水平分别为 ( 13 3± 13 8)ng/L、( 5 0 5± 2 5 8)ng/L ,明显高于正常非孕组的 ( 4 6± 6 4 )ng/L、( 2 0 3± 2 8 2 )ng/L(P <0 0 1) ;IL 4、IL 10的水平分别为 ( 13 8± 1 0 )ng/L、( 13 5± 0 7)ng/L ,明显低于正常非孕组的 ( 14 5± 1 2 )ng/L、( 14 9± 2 4 )ng/L(P <0 0 1) ;γ干扰素、转化生长因子 β1的水平分别为 ( 4 5 4± 10 4 8)ng/L、( 3 1 8± 2 6 6) μg/L ,与正常非孕组的 ( 15 2±3 6)ng/L、( 3 0 3± 2 7 9) μg/L比较 ,差异无显著性 (P >0 0 5 )。 ( 2 )流产组治疗后血清IL 2 [( 5 6±9 0 )ng/L]、IL 12 [( 2 8 5± 4 0 3 )ng/L]水平 ,较治疗前明显降低 (P <0 0 1) ;IL 4 [( 14 7± 1 2 )ng/L]、IL 10 ( 15 0± 1 8)ng/L]水平 。Objective To investigate the changes of serum T helper cell (TH)1/TH2 type cytokines after the active immunotherapy in unexplained habitual abortion (UHA) women. Methods Concentrations of interleukin (IL) 2, IL 12, interferon (IFN) γ, IL 4, IL 10 and transforming growth factor (TGF) β1 were measured by enzyme linked immunosorbant assay (ELISA) method in sera from thirty three cases of unexplained habitual abortion (UHA) women before and after active immunotherapy. Thirty normal non pregnancy (NNP) women and thirty normal pregnancy (NP) women were taken as control. Results (1) Serum concentrations of IL 2 and IL 12 were higher significantly (P<0 01) in UHA women [(13 3±13 8) ng/L and (50 5±25 8) ng/L, respectively] than those in NNP women [(4 6±6 4) ng/L and (20 3±28 2) ng/L, respectively]. Serum concentrations of IL 4 and IL 10 were lower significantly (P<0 01) in UHA women [(13 8±1 0) ng/L and (13 5±0 7) ng/L,respectively] than those in NNP women [(14 5±1 2) ng/L and (14 9±2 4) ng/L, respectively]. However, there were no significant differeces in serum concentrations of IFN γ and TGF β1 between UHA women and NNP women. (2) Serum concentrations of IL 2 were lower significantly (P <0 05) in NP women [(1 6±4 3) ng/L] than those in NNP women. Serum concentrations of IL 4 were higher significantly (P <0 01) in NP women [(16 3±0 8) ng/L] than those in NNP women. But there were no significant differences in serum concentrations of IFN γ, IL 10 and TGF β1 between NP and NNP women. (3) After activeimmunotherapy, serum concentrations of IL 2 and IL 12 were decreased significantly [(5 6±9 0) ng/L and (28 5±40 3) ng/L respectively, P <0 01] whereas serum concentrations of IL 4 and IL 10 were increased significantly [(14 7±1 2) ng/L and (15 0±1 8) ng/L respectively, p <0 01 ] in UHA women. In addition, no significant changes in serum concentrations of IFN γ and TGF β1 were found in UHA women after i

关 键 词：习惯性流产 免疫疗法 TH1细胞 TH2细胞 细胞活素类 

分 类 号：R714.21[医药卫生—妇产科学] R459.9[医药卫生—临床医学]