马桑内酯对大鼠海马脑片细胞外Na^+、K^+活度的影响  

The Effect of Coriaria Lactone on Extracellular Sodium and Potassium Ionic Activities of Hippocampal Slice in Rat

作　　者：邹飞[1] 倪再玉[2] 袁太宁[1] 王尊琼 郑世玲[1] 王阿敬[3] 

机构地区：[1]三峡大学医学院生理教研室,宜昌443003 [2]三峡大学医学院药理教研室,宜昌443003 [3]华中科技大学同济医学院实验医学研究中心,武汉430030

出　　处：《湖北民族学院学报（医学版）》2001年第2期8-10,共3页Journal of Hubei Minzu University(Medical Edition)

基　　金：国家自然科学基金资助项目 (39330 2 10 )

摘　　要：目的　探讨癫痫过程中神经细胞Na+ 、K+ 通透性的变化。方法　应用Na+ 、K+ 选择性微电极检测了马桑内酯 (5 μl,5× 10 -4 mol/L)灌流的大鼠海马脑片神经细胞外Na+ 、K+ 活度的改变。结果　马桑内酯灌流海马脑片 30s后可观察到海马神经细胞外Na+ 活度明显下降 ,与对照组比较下降幅度为 10mmol/L(P <0 .0 1) ,在实验观察的时间内 (2 0min)未见Na+ 活度的恢复 ;海马神经细胞外K+ 活度在灌流后 1min、2min、3min、5min和 10min分别高于对照组 3.3mmol/L、4.8mmol/L、4.9mmol/L、6 .5mmol/L和 8.8mmol/L ,在以后 10min内K+ 活度亦未见恢复。结论　马桑内酯作用于离体海马神经细胞时存在Na+ 内流、K+Objective To study the changes of ionic activity of extracellular sodium and potassium (o?o) of hippocampal slice perfused by coriaria lactone (CL).Methods The sodium ion (Na +) and potassium ion (K +) selective microeletrodes were used to measure alteration of the o and the o of perfused hippocampal slice with CL. Results 30s after perfusing of CL into hippocampal slice, the o decreased 10 mmol/L compared with control values ( P <0.01) and did not return to the control level during the experiment (20min) .1min ?2min ?3min ?5min and 10min after perfusing of CL into hippocampal slice,The o increased 3.3mmol/L ?4.8mmol/L ?4.9mmol/L? 6.5mmol/L and 8.8 mmol/L respectively compared with control values ( P <0.01), 10 min after, the o always kept the level. Conclusion: The influx of Na + and the flux of K + occurred during perfusing hippocampal slice with CL .

关 键 词：马桑内酯 海马脑片 NA^+ K^+ CL 癫痫 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R742.1[医药卫生—基础医学]