Balb/C鼠尾胶原与大鼠尾胶原对新生Balb/C鼠脑细胞培养的影响  

Effects of Balb/C mouse tail collagen and rat tail collagen on newborn Balb/C mouse brain cells in culture

作　　者：赵兰娟[1] 龚蕴贞[1] 林维芬[1] 胡涛[1] 张咏南[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2001年第2期90-92,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教委自然科学基金!资助课题 (编号 :JL0 0 69)

摘　　要：目的　确定鼠脑细胞体外培养的适宜生长基质。方法　以Balb/C鼠尾胶原与大鼠尾胶原作为生长基质 ,培养新生Balb/C鼠脑细胞。结果　Balb/C鼠尾胶原有利于神经元的生存、生长与分化 ,而大鼠尾胶原则利于星形胶质细胞的生存、生长、分化与增殖。结论　 2种鼠尾胶原均为培养鼠脑细胞适宜的生长基质 ,培养Objective To determine the suitable growth substrate for brain cells in culture. Methods Two kinds of collagen from Balb/C mouse tail and rat tail respectively as growth substrate was applied to culturing newborn Balb/C mouse brain cells. Results Balb/C mouse tail collagen was suitable to the survival, growth, differentiation of neurons, whereas rat tail collagen those of astrocytes. Conclusion Two kinds of collagen are suitable as growth substrate of neuron culture. Balb/C mouse tail collagen may be applied as growth substrate for short term cultures, whereas rat tail collagen may be adopted for long term cultures.

关 键 词：神经元 大鼠 SPRAGUE-DAWLEY 近交BALBC 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]