同种抗原特异性T淋巴细胞克隆方法的建立  

Establishment of method of cloning alloantigen-specific T lymphocyte

作　　者：唐功耀[1] 乔军[2] 赵武述[2] 

机构地区：[1]中日友好医院肾内科,北京100029 [2]中日友好临床医学研究所免疫室,北京100029

出　　处：《安徽医科大学学报》2001年第2期107-110,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的　建立抗原特异性T淋巴细胞克隆的方法。方法　以供体脾细胞作同种抗原 ,刺激受体外周血单个核细胞 ,用有限稀释法进行T淋巴细胞克隆。结果　获得 3个T淋巴细胞株。经免疫组化分析 ,有 2个细胞株为CD3+CD4-CD8+ 。另一个细胞株为CD3+ CD4-CD8-。后者经流式细胞仪分析纯度为 97%。抗原特异性检测表明 :用供体细胞刺激 ,克隆T细胞发生增殖作用 ;用无关个体细胞刺激 ,无增殖作用。结论　成功地建立了抗原特异性T淋巴细胞克隆方法。Objective To establish the method of alloantigen specific T lymphocyte clones. Methods\ PBMC of the recipient was stimulated by donor splenocytes for twice and the stimulated T lymphocytes were cloned by limited dilution. Results\ Three clones of T lymphocytes were obtained. By analysis of immunohistochemistry, two of the three clones were CD3 +CD4 -CD8 +, another one was CD3 +CD4 -CD8 -. The latter was analyzed by flowcytometry and CD3 + was 98 87%, CD4 - 97 05%, CD8 - 97 14%. Antigen specificity of the cloned T lymphocytes was determined by stimulation of donor splenocytes and non related individual splenocytes. When stimulated by donor splenocytes, the cloned T lymphocytes proliferated and did not show proliferative role when stimulated by non related individual splenocytes. Conclusion\ Our method of cloning alloantigen specific T lymphocytes is successful.

关 键 词：表位 T淋巴细胞 克隆细胞 

分 类 号：R392.12[医药卫生—免疫学]